Spatial transcriptomics, a novel technology to map the transcriptional architecture, is technically limited to discover T cell receptor (TCR) sequences as the 3' approach lacks sufficient coverage. Here, we established SPTCR-seq, a method to capture TCR sequences followed by long-read sequencing ...
In this study, we utilized spatially resolved T cell receptor sequencing (SPTCR-seq) to investigate T cell responses in GBM, a “cold” tumor with overall low T cell abundance. Through target enrichment, long-read sequencing, and read consensus correction, we gained insights into the spatial h...