scTCR-seq分析: MIXCR + scRepertoire 重拾生活信心关注赞赏支持scTCR-seq分析: MIXCR + scRepertoire 重拾生活信心关注IP属地: 上海 2024.05.07 16:34:42字数14阅读287 https://www.borch.dev/uploads/screpertoire/ rm(list=ls()) #devtools::install_github("ncborcherding/scRepertoire") # Load the ...
We describe a new pipeline, 'RM-scTCR-Seq', which, for the first time, enables RM specific single cell TCR amplification, reconstruction and pairing of RM TCR's with their transcriptional profiles. We apply this method to a RM model of GVHD, and identify and t...
Subsequently, we cloned five and four TCRs from YTN2 and YTN16, respectively, based on scTCR-Seq data. The cloned TCRs were retrovirally transduced into TG40 cells, and their reactivity to tumor cells and the three neoAgs (mCdt1, mScarb2 and mZfp106) was assessed. Furthermore, the ...