图6. 空间转录组测序技术的原理 该论文的通讯作者Joakim Lundeberg也是瑞典Spatial Transcriptomics公司的联合创始人之一,2018年底10X Genomics宣布收购Spatial Transcriptomics,并于2019年发布Visium空间基因表达解决方案(Visium Spatial Gene Expression Solution)。 02、10X Genomics Visium空间转录组技术 1、技术原理 将冰冻组...
今年3月 Nature子刊《Nature Methods》发表了一篇关于空间转录组学的全面综述:Museum of spatial transcriptomics,作者对1987年以来有关空间转录组学的文献进行了整理回顾,并对该领域的趋势进行了深入分析,如…
Transcriptomic analysis only reveals one aspect of cellular function. Spatial multi-omics is a technique that combines spatial transcriptomics with other omics modalities to reveal the multiple layers of cell function. Several methods have been developed to achieve comprehensive spatially resolved molecular ...
Its genuinely a Heisenbergian tradeoff that baffled the biologist for a long time until now; an innovative solution seems to be in place. Spatial transcriptomics (ST) is the perfect marriage between localization and quantitation without compromising either. It is a method that...
“Spatial transcriptomics will be a key part of how we arrive at the ‘Holy Grail’ of biology: Full 3D models of cells, organs and even organisms.” Pascal Nordgren Timshel, ZS Discovery “Spatial transcriptomics will be a key part of how we arrive at the ‘Holy Grail’ of biology: Ful...
Although bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) have provided insights into cell types and heterogeneity in the respiratory system, the relevant specific spatial localization and cellular interactions have not been clearly elucidated. Spatial transcriptomics (ST) has filled this ...
Fig. 1: Spatial transcriptomics resolves the engram assembly and memory-associated genes. a, Experimental scheme for tracing engram cells in a fear conditioning model. Active cells during the return phase were permanently tagged with tdTomato and used for differential analyses of engram cells. 4-OHT...
we provide a foundation for systematic mapping of functional ensembles and circuits selectively engaged in various cognitive and executive functions of PFC. Spatial transcriptomics is a rapidly developing field60, and similar to recent studies25,27,61, MERFISH enabled systematic decoding of PFC’s molec...
High-definition spatial transcriptomics (HDST), a method similar to Slide-seq in its use of a barcoded bead array, allows for higher resolution by using beads that are smaller than single cells. The barcoded beads are enriched with poly(dT) nucleotides and deposited into 2μm wells to obta...
The power of spatial transcriptomics Discover what's possible with spatial transcriptomics, including the additional depth it can add to single cell research projects, through these high-impact publications. Read now Human liver cancer; Visium Spatial ...