图6. 空间转录组测序技术的原理 该论文的通讯作者Joakim Lundeberg也是瑞典Spatial Transcriptomics公司的联合创始人之一,2018年底10X Genomics宣布收购Spatial Transcriptomics,并于2019年发布Visium空间基因表达解决方案(Visium Spatial Gene Expression Solution)。 02、10X Genomics Visium空间转录组技术 1、技术原理 将冰冻组...
图6. 空间转录组测序技术的原理 该论文的通讯作者Joakim Lundeberg也是瑞典Spatial Transcriptomics公司的联合创始人之一,2018年底10X Genomics宣布收购Spatial Transcriptomics,并于2019年发布Visium空间基因表达解决方案(Visium Spatial Gene Expression Solution)。 02、10X Genomics Visium空间转录组技术 1、技术原理 将冰冻组...
今年3月 Nature子刊《Nature Methods》发表了一篇关于空间转录组学的全面综述:Museum of spatial transcriptomics,作者对1987年以来有关空间转录组学的文献进行了整理回顾,并对该领域的趋势进行了深入分析,如…
空间转录组学技术高分文章集锦 空间转录组学(Spatial Transcriptomics)是在二维平面上定位 mRNA-seq 数据的技术。常规的转录组测序可以揭示 mRNA 的表达信息,但无法获得准确定位;常规的染色显影技术以揭示特定组织区域的分子特征,但不包含准确、完整的 mRNA 表达谱信息。为了弥补这个技术缺憾,在一次实验中同时获取 RNA 的...
and interactions between cells. Spatial transcriptomics (ST) is a method that maps gene expression in tissue while preserving spatial information. It can reveal cellular heterogeneity, spatial organization and functional interactions in complex biological systems. ST can also complement and integrate with ...
这项技术的通讯作者Joakim Lundeberg也是瑞典Spatial Transcriptomics公司的联合创始人之一。2018年底，10X Genomics宣布收购Spatial Transcriptomics，并于2019年发布Visium空间基因表达解决方案。Visium空间转录组技术采用独特的技术原理，将冰冻组织切片放置在芯片的捕获区域内，通过HE染色和成像后进行透化处理，释放...
Spatial transcriptomics technologies are assessed by key metrics, such as resolution of the spatial biology, gene plexity (the number of genes detected), and gene sensitivity (the linear dynamic range of accurate transcript detection). 10x Genomics spatial tools are optimized to provide robust insight...
Spatial transcriptomics (ST) is an emerging field that combines spatial information with transcriptomic data to provide a more comprehensive understanding of gene expression within the context of tissue architecture. This innovative approach has revolutionized the study of complex biological systems, allowing...
Spatial transcriptomics (ST) has filled this gap and has been widely used in respiratory studies. This review focuses on the latest iterative technology of ST in recent years, summarizing how ST can be applied to the physiological and pathological processes of the respiratory system, with emphasis...
Fig. 1: Spatial transcriptomics resolves the engram assembly and memory-associated genes. a, Experimental scheme for tracing engram cells in a fear conditioning model. Active cells during the return phase were permanently tagged with tdTomato and used for differential analyses of engram cells. 4-OHT...