typically obtained from biopsies, are not sufficiently large to support multi-omics analysis; (2) The majority of the tissue is composed of normal epithelia (NE),
图6. 空间转录组测序技术的原理 该论文的通讯作者Joakim Lundeberg也是瑞典Spatial Transcriptomics公司的联合创始人之一,2018年底10X Genomics宣布收购Spatial Transcriptomics,并于2019年发布Visium空间基因表达解决方案(Visium Spatial Gene Expression Solution)。 02、10X Genomics Visium空间转录组技术 1、技术原理 将冰冻组...
To address this loss of spatial resolution, we established an analysis tool (Spatial Transcriptomics ANaLYsis (STANLY) that aligns dots from multiple samples from different animals into one common anatomical reference space, the Common Coordinate Framework (CCF) of the Allen Mouse Brain Atlas31, thus...
Transcriptomic analysis only reveals one aspect of cellular function. Spatial multi-omics is a technique that combines spatial transcriptomics with other omics modalities to reveal the multiple layers of cell function. Several methods have been developed to achieve comprehensive spatially resolved molecular ...
Discover what's possible with spatial transcriptomics, including the additional depth it can add to single cell research projects, through these high-impact publications. Read now Human liver cancer; Visium Spatial Zhang S, et al. Spatial transcriptomics analysis of neoadjuvant cabozantinib and nivolumab...
今年3月 Nature子刊《Nature Methods》发表了一篇关于空间转录组学的全面综述:Museum of spatial transcriptomics,作者对1987年以来有关空间转录组学的文献进行了整理回顾,并对该领域的趋势进行了深入分析,如…
单细胞转录组测序技术可以说是融合了高通量组学技术和传统的单细胞研究手段,即解决了通量和分辨率的问题。空间转录组技术(spatial transcriptomics)则需要利用常规的原位技术和组学技术两方面的优势。 图3. 单细胞测序技术解决了通量和分辨率的问题 现有的空间转录组技术主要分为两类:一类是基于杂交和成像的方法,例如smFI...
In summary, SpaDo stands out as a pioneering framework for multi-slice spatial domain analysis in spatial transcriptomics. Its superior performance in detecting multi-slice spatial domains, providing reference-based spatial domain annotation, and conducting multi-slice clustering analysis addresses the limi...
Spatial transcriptomics technologies developed in recent years can provide various information including tissue heterogeneity, which is fundamental in biological and medical research, and have been making significant breakthroughs. Single-cell RNA sequen
Genome-wide spatial transcriptomics analysis provides an unprecedented approach to untangle the dysregulated cellular network in the vicinity of pathogenic hallmarks of AD and other brain diseases. Graphical Abstract Download: Download high-res image (323KB) Download: Download full-size image...