Small intestine organoidsSmall intestinal organoids have become an important tool to study crypt homeostasis, cell fate dynamics and tissue biomechanics. Yet, the mechanisms that drive the budding of crypts from the smooth organoid epithelium remain incompletely understood. Locally enhanced proliferation ...
Zhang Y et al (2021) 3D organoids derived from the small intestine: an emerging tool for drug transport research. Acta Pharm Sin B 11(7):1697–1707 Google Scholar Tian CM et al (2023) Stem cell-derived intestinal organoids: a novel modality for IBD. Cell Death Discov 9(1):255 Goog...
InflammationWound healingThe gastrointestinal tract (GIT), in particular, the small intestine, plays a significant role in food digestion, fluid and electrolyte transport, drug absorption and metabolism, and nutrient uptake. As the longest portion of the GIT, the small intestine also plays a vital ...
Different adhesion proteins impact the small intestinal organoid-derived epithelial cells ability to adhere and grow as monolayers Next, we wanted to assess the effect of different adhesion proteins and mixtures thereof on the ability of small intestine organoid-derived epithelial cells to adhere to and...
For cell culture, small intestine organoids derived from the intestinal stem cells were cultured alone or co‐cultured with macrophages (VDR+/+ or VDR/). After 3 days, the organoids and organoid‐macrophage co‐cultures were collected for western blots. Western blots were used to measure and ...
7d). This is consistent with prior work, which has shown that Her2 signaling is primarily responsible for maintaining ERK activity in 3-dimensional small intestine organoids43. Together, our data reveal that the large intestine is more reliant on EGFR-induced ERK phosphorylation and point to ...
we show thatCryptosporidiumcan infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of theCryptosporidiumtranscriptome during organoid infection reveals dynamic regulation of transcripts rela...
The small intestine is the longest section of the gut and is composed of the duodenum, the jejunum and the ileum, and is separated from the stomach by the pyloric sphincter. From: Dictionary of Toxicology (Third Edition), 2015 About this pageSet alert ...
2.3. Culture of mouse small intestine organoids Isolated crypts and villi from CTRL mice were filtered through a 70 μm filter (VWR International, 2572267) to retain crypts. After centrifugation of the suspension at 200×g for 5 min, 250 crypts were seeded in 25 μL Matrigel (Corning, 3542...
Small IntestineShort Bowel SyndromeOrganoid UnitStem CellRegenerationShort bowel syndrome (SBS) results from the loss of a highly specialized organ, the small intestine. SBS and its current treatments are associated with high morbidity and mortality. Production of tissue-engineered small intestine (TESI...