Human small intestinal organotypic culture model for drug permeation, inflammation, and toxicity assaysIntestinal tissue modelDrug absorptionMetabolismDrug-induced toxicityNanotoxicityInflammationWound healingThe gastrointestinal tract (GIT), in particular, the small intestine, plays a significant role in food ...
For cell culture, small intestine organoids derived from the intestinal stem cells were cultured alone or co‐cultured with macrophages (VDR+/+ or VDR/). After 3 days, the organoids and organoid‐macrophage co‐cultures were collected for western blots. Western blots were used to measure and ...
7d). This is consistent with prior work, which has shown that Her2 signaling is primarily responsible for maintaining ERK activity in 3-dimensional small intestine organoids43. Together, our data reveal that the large intestine is more reliant on EGFR-induced ERK phosphorylation and point to ...
Intestinal organoid cultures were prepared from the mouse small intestine according to previously published protocols. We starved the organoids for various time periods of endogenous EGF and perturbed the pathway with inhibitors and growth factors. The levels of phospho-ERK (pERK) were determined using...
Why is developing a small-intestine organoid helpful in this context? Organoids are essential because it’s impossible to grow most types of enteroendocrine cells in a standard culture. Organoids allow you to grow and enrich cells within their physiological context, and other groups have developed...
Organoids were plated in 50 μl matrigel beads in 24 well plates. Complete culture media is composed of advanced DMEM/F12 (Life Technologies), 1X Glutamax (Life Technologies), 1X HEPES buffer (Life Technologies), 1X Pen/Strep (Life Technologies), 1X N2 supplement (Life Technologies), 1...
Circadian rhythms in primary or passaged PER2::LUC jejunal organoids were similarly robust; they also synchronized upon serum shock and persisted beyond 2 weeks in culture. Remarkably, unshocked organoids autonomously synchronized rhythms within 12 hours of recording. The onset of this autonomous ...
However, because the sophistication of intestine organoids has improved, with them now incorporating functioning nerves, immune cells, and other essential functions, they have huge potential to be a form of cell therapy transplantation. Cincinnati Children’s has invested over $10 million as part of...
Using a culture system that mimics the native intestinal epithelial stem cell niche, these cells are capable of generating “organoids” that contain all four epithelial cell types of the small intestinal epithelium. Furthermore, the Sox9(EGFP) multipotent intestinal epithelial stem cells express CD...
the intestinal organoid culture system comprised only of epithelial cells is short of recapitulating the intricate cross-talks among epithelial cells, immune cells, stromal cells, and nerve cells that are present in the intact small intestine. Thus, it is highly desirable to develop a reliable met...