Zhang Y et al (2021) 3D organoids derived from the small intestine: an emerging tool for drug transport research. Acta Pharm Sin B 11(7):1697–1707 Google Scholar Tian CM et al (2023) Stem cell-derived intestinal organoids: a novel modality for IBD. Cell Death Discov 9(1):255 Goog...
Human small intestinal organotypic culture model for drug permeation, inflammation, and toxicity assaysIntestinal tissue modelDrug absorptionMetabolismDrug-induced toxicityNanotoxicityInflammationWound healingThe gastrointestinal tract (GIT), in particular, the small intestine, plays a significant role in food ...
For cell culture, small intestine organoids derived from the intestinal stem cells were cultured alone or co‐cultured with macrophages (VDR+/+ or VDR/). After 3 days, the organoids and organoid‐macrophage co‐cultures were collected for western blots. Western blots were used to measure and ...
7d). This is consistent with prior work, which has shown that Her2 signaling is primarily responsible for maintaining ERK activity in 3-dimensional small intestine organoids43. Together, our data reveal that the large intestine is more reliant on EGFR-induced ERK phosphorylation and point to ...
Intestinal organoid cultures were prepared from the mouse small intestine according to previously published protocols. We starved the organoids for various time periods of endogenous EGF and perturbed the pathway with inhibitors and growth factors. The levels of phospho-ERK (pERK) were determined using...
Why is developing a small-intestine organoid helpful in this context? Organoids are essential because it’s impossible to grow most types of enteroendocrine cells in a standard culture. Organoids allow you to grow and enrich cells within their physiological context, and other groups have developed...
Organoids were plated in 50 μl matrigel beads in 24 well plates. Complete culture media is composed of advanced DMEM/F12 (Life Technologies), 1X Glutamax (Life Technologies), 1X HEPES buffer (Life Technologies), 1X Pen/Strep (Life Technologies), 1X N2 supplement (Life Technologies), 1...
The dataset was merged with published data from native human intestine (Hickey et al., 2023) and organoids (Beumer et al., 2020), which contained fewer EECs, revealing good overlap between EEC clusters from the different studies. Methods Human organoid culture and generation of CHGA-Venus ...
culture system. Here, we show thatCryptosporidiumcan infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of theCryptosporidiumtranscriptome during organoid infection reveals dynamic ...
In mouse small intestine organoids, p38 is activated during crypt formation and differentiation in responses to ROS signaling [66]. Furthermore, impairing OXPHOS in mouse small intestine organoids, by blocking ETC complexes or treating them with antioxidants, altered crypt formation [61]. Taken ...