Adult-stem-cell-derived organoids model human epithelial tissues ex vivo, which enables the study of host–microbe interactions with great experimental control. This protocol comprises methods to coculture organoids with microbes, particularly focusing on human small intestinal and colon organoids exposed...
Intestinal organoid cocultures with microbes.doi:10.1038/S41596-021-00589-ZJens PuschhofCayetano Pleguezuelos-ManzanoAdriana Martinez-SilgadoNinouk AkkermanAurelia SaftienCharelle BootAmy de WaalJoep BeumerDevanjali DuttaInha HeoNature Publishing Group...
Intestinal organoid cocultures with microbes. Nat Protoc. 2021;16(10):4633–49. Article CAS PubMed Google Scholar Holokai L, Chakrabarti J, Broda T, Chang J, Hawkins JA, Sundaram N, Wroblewski LE, Peek RM, Wang J, Helmrath M, et al. Increased programmed death-ligand 1 is an early ...
Lung Organoids: Systematic Review of Recent Advancements and its Future Perspectives T. Thangam Krupakar Parthasarathy Sakthivel Jayaraj Tissue Engineering and Regenerative Medicine(2024) Associated content Intestinal organoid cocultures with microbes
Define intestinal crypts. intestinal crypts synonyms, intestinal crypts pronunciation, intestinal crypts translation, English dictionary definition of intestinal crypts. adj. Of, relating to, or constituting the intestine: the intestinal wall; intestinal
Coculture with other cell types, such as immune cell subtypes or endothelial cells, is also difficult given that organoids are typically embedded in a 3-dimensional matrix. One potential way to overcome such challenges is to combine intestinal organoid culture with microengineering technology. Small ...
T3 can control cell proliferation in organoid cultures. Organoids treated with T3 have longer and larger buds and faster cell cycles, resulting in the earlier emergence of buds during in vitro growth. The loss of ISCs and their differentiation to intestinal secretory cells can increase the turn...
Similarly, using in vitro cell cultures of intestinal organoids, we show that exposure of IECs to IFNγ decreases cell survival and IL-10R expression. Adding IL-10 together with IFNγ only partially rescues cell survival. Thus, these results propose for the first time that IFNγ inhibits IL-...
Intestinal organoids for assessing nutrient transport, sensing and incretin secretion. Sci. Rep. 5, 16831 (2015). CAS PubMed PubMed Central Google Scholar Puschhof, J. et al. Intestinal organoid cocultures with microbes. Nat. Protoc. 16, 4633–4649 (2021). CAS PubMed Google Scholar Co...
The organoid pellet was resuspended in 1 mL TrypLE Express and incubated at 37 °C for 10 min with shaking. To stop the TrypLE action, 6 mL of DMEM/F12 was added to the mixture before centrifugation at 100× g for 5 min at 4 °C. The supernatant was removed, and the pellet was ...