Powell RH, Behnke MS. 2017. WRN conditioned media is sufficient for in vitro propagation of intestinal organoids from large farm and small companion animals. Biology Open 6:698-705. DOI: https://doi.org/10. 1242/bio.021717, PMID: 28347989...
Organoids were obtained as previously described.50 Briefly, after cleaning, whole colons were cut into pieces, washed and incubated with 1 mg/ml collagenase D and 100 μg/ml dispase II for 1 h, then organoids were purified by successive sedimentation steps in 2% (w/v) sorbitol-HBSS. ...
2. Homeostasis within the stem-cell compartment is governed by positive- and negative-feedback regulation of instructive extrinsic and intrinsic signals3,4. ErbB signalling is a prerequisite for maintenance of the intestinal epithelium following injury and tumour formation...
Organoids were obtained as previously described.50Briefly, after cleaning, whole colons were cut into pieces, washed and incubated with 1 mg/ml collagenase D and 100 μg/ml dispase II for 1 h, then organoids were purified by successive sedimentation steps in 2% (w/v) sorbitol-HBSS....
Organoids were obtained as previously described.50Briefly, after cleaning, whole colons were cut into pieces, washed and incubated with 1 mg/ml collagenase D and 100 μg/ml dispase II for 1 h, then organoids were purified by successive sedimentation steps in 2% (w/v) sorbitol-HBSS....