We investigated dynamic feature of immune cells over the Belimumab treatment using longitudinal single-cell transcriptome data. Methods We conducted single-cell RNA sequencing (scRNA-seq) along with T cell receptor (TCR) and B cell receptor (BCR) repertoire from serial longitudinal PBMC samples (0,...
This overlap complicates early diagnosis and accurate treatment.#The transcriptomic profiling of peripheral blood mononuclear cells (PBMCs) from MCTD patients was performed using both bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) for the first time. Additionally, we applied MCTD ...
2.4 Cell sorting and RNAseq 2.4.1 SortingPBMCs from 12 JSLE patients were washed in MACS buffer (1X PBS (Sigma), 2% FBS (Labtech) and 1mM EDTA (Sigma)) and stained with Zombie NIR™ Fixable Viability Kit (Biole...
the New York University Genome Technology Center for Illumina sequencing, the Yerkes Genomics Core for RNA-seq library preparation, the Emory Pediatrics Flow Cytometry core for flow cytometry isolation of cell subsets and the Emory Integrated Genetics and Computational Core for Bioanalyzer and sequencing...
RNA-Seq data43 were downloaded from the eQTL resources from the Gilad/Pritchard group website. This gene expression data was collected 69 LCLs generated as part of the International HapMap project. The cell lines were derived from primary cells of YRIs from Ibadan, Nigeria. Genotype data in th...
Left: RNA-seq data was generated from poly(A)+ RNA-seq (black) and poly(A) RNA-seq (red) in H9 cells. SLERT (red) is ended with SNORA5A and SNORA5C. Right: NB detected SLERT in H9 and PA1 cells. See also Figures S1A–S1C. (B) Recapitulation of SLERT processing. Top: a...
Methods: Using single-cell RNA sequencing (scRNA-seq), we profiled 68,494 peripheral blood mononuclear cells (PBMC) from four SLE patients with and without CAD. The data were analyzed using Seurat pipelines. We identified differentially expressed genes (|log2(fold change) |>0.5, p adj<0.05)...
Several of these technologies are becoming firmly established in the SLE field, including single cell RNA Seq of blood and dissociated kidney using recently-developed methods in several consortia related to SLE and cancer; low input RNA-Seq of bulk purified cells; Assay of Transposase Accessible ...
microRNAmiRNA serum levelsMCTDACTDMixed connective tissue disease (MCTD) is a rare disorder characterized by symptoms that overlap two or more Autoimmune Connective Tissue Diseases (ACTDs). The aim of this study was to determine whether miRNAs participating in the TLRs signaling pathway could serve ...
We took advantage of this bimodal expression using single cell technology to perform RNA-seq analyses of ARID3a+ and ARID3a SLE B cells to identify differentially expressed genes associated with ARID3a. PCA analyses of scRNA seq data show that ARID3a+ nave B cells cluster together and away ...