Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
Our results demonstrate that single-nuclei 3' or 5'-RNA-sequencing data can be used to identify known somatic variants in single-nuclei when they are expressed within proximity to a transcript end.doi:10.1038/s41598-023-27700-6Sydney E. Townsend...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
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Single-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nuclei isolation combined with nanowell-based library preparation. Our method enables the transcriptome analysis of thousands of individual plant...
在一篇发表于《RAPID COMMUNICATION》的“单细胞核RNA测序比单细胞RNA测序可以发现更多小鼠肾脏纤维细胞稀少的细胞类型和未知的细胞状态”文章中提到,采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核...
(fair) representation of the nuclei from all of the donors, a property required by many single-nuclei downstream analyses. We then employed this approach to process the snuclRNA-seq from human frozen brains and analyze the distribution of neuronal subtypes in the brains with Mendelian and ...
Delete reference_data directory Oct 24, 2023 3e93191·Oct 24, 2023 History 139 Commits R man vignettes DESCRIPTION LICENSE NAMESPACE README.md DeTREMis a modification of the MuSiC deconvolution algorithm optimized for single-nuclei reference data. ...
UsingsinglenucleiforRNA-seqtocapturethetranscriptomeof postmortemneurons SugunaRaniKrishnaswami 1,9 ,RashelVGrindberg 2,9 ,MarkNovotny 1 ,PratapVenepally 3 , BenjaminLacar 4 ,KunalBhutani 1 ,SaraBLinker 4 ,SonPham 4 ,JenniferAErwin 4 ,JeremyA Miller 5 ,RebeccaHodge 5 ,JamesKMcCarthy 1 ,Marti...
nuclei from distinct samples. Comparing human brain cortex samples profiled with or without hashing antibodies, we demonstrate that nucleus hashing does not significantly alter recovered profiles. We develop DemuxEM, a computational tool that detects inter-sample multiplets and assigns singlets to their...