W. Single-molecule protein sensing in a nanopore: a tutorial. Chem. Soc. Rev. 47, 8512–8524 (2018). CAS PubMed PubMed Central Google Scholar Firnkes, M., Pedone, D., Knezevic, J., Döblinger, M. & Rant, U. Electrically facilitated translocations of proteins through silicon nitride...
Herein, we addressed these outstanding issues to the nanopore single-molecule biosensor, which provided an effective platform for protein denaturation in near-real physical environment, and particularly collected data based on individual difference instead of ensemble average. Nanopore sensing is a powerful...
& Joo, C. Single-molecule protein sequencing through fingerprinting: computational assessment. Phys. Biol. 12, 055003 (2015). Article Google Scholar Ohayon, S., Girsault, A., Nasser, M., Shen-Orr, S. & Meller, A. Simulation of single-protein nanopore sensing shows feasibility for whole...
NanoporeElectrokineticsSingle-molecule sensingNanofluidicsSilicon nitrideCarbohydrateA nanopore can be fairly鈥攂ut uncharitably鈥攄escribed as simply a nanofluidic channel through a thin membrane. Even this simple structural description holds utility and underpins a range of applications. Yet significant ...
protein trappingSingle﹎olecule sensing technologies aim to detect and characterize single biomolecules, but generally need labeling while the measurement times and throughput are severely restricted by a lack of positional control over the molecule. Here, a plasmonic nanopore biosensor is reported where ...
Nanopore sensing is a key technology for single-molecule detection and analysis. Solid-state nanopores have emerged as a versatile platform, since their fa... G Lanzavecchia,A Sapunova,A Douaki,... 被引量: 0发表: 2024年 加载更多来源期刊 Physics Today 研究点推荐 nanopores Single-molecule sen...
Imec's aim tomass-produce stable and reliable solid-state nanoporesis key for the field of single-molecule sensing. The ability to produce sub-5nm nanopores with uniform size and high stability in wet environments overcomes one of the biggest hurdles in nanopore technology. This advancement ...
Single-molecule nanopore sensing of actindynamics and drug binding†Xiaoyi Wang, ‡ a Mark D. Wilkinson, ‡ a Xiaoyan Lin,aRen Ren,aKeith R. Willison,aAleksandar P. Ivanov,aJake Baum* band Joshua B. Edel* aActin is a key protein in the dynamic processes within the eukaryotic cell....
Nanopore sensing is highly dependent on the pore shape. We reported an MspA protein sensor that is very suitable for detecting small molecules. By the host-guest response of am7-β-CD lodged in the negatively charged β barrel constriction of MspA, single-molecule analysis could be easily perf...
We study the translocation dynamics of a single protein molecule attached to a double-stranded DNA that is threaded through a solid-state nanopore by optical tweezers and an electric field (nanopore force spectroscopy). We find distinct asymmetric and retarded force signals that depend on the protei...