In nanopore sequencing of proteins, the amino acid sequence is determined by measuring the ionic current through a nanopore in a thin membrane as the protein chain permeates through the nanopore. The computational study by A. Aksimentiev and co-workers, presented on page 4830, explores the ...
Protein sequencing and the identification of post-translational modifications are key to understanding cellular signalling pathways and metabolic processes in health and disease. Nanopores, that is, nanometre-sized holes in a membrane, were previously put to use for DNA and RNA sequencing, offering sing...
Nanopores for sequencing proteins Developments in nanopore-based peptide detection and sequencing show promise of a breakthrough. Arunima Singh Comments & Opinion06 Dec 2023Nature Methods Volume: 20, P: 1870 Nanoturbine driven by flow across a nanopore ...
It is known that proteins can distinguish closely similar molecules. Moreover, several proteins can work as biological nanopores in order to perform single molecule detection and sequencing. Unfortunately, while DNA sequencing by means of nanopores is demonstrated, very few examples of nanopores able ...
Nanopore sequencing of DNA-barcoded probes for highly multiplexed detection of microRNA, proteins and small biomarkers This study establishes a method for multiplexed detection of up to 40 different biomarkers in a single assay by combining nanopore sequencing with analyte-selective barcoded probes, inc...
Nanopore-based sensing is alabel-free techniqueand requires no amplification steps which is for example beneficial when determining an exact viral load in a blood sample or parsing through a sample made up of proteins. ForDNA and RNA sequencing, there are already nanopore-based commercial appli...
Nanopore-based sensing is a powerful technique for the detection of diverse organic and inorganic molecules, long-read sequencing of nucleic acids, and single-molecule analyses of enzymatic reactions. Selected from natural sources, protein-based nanopores enable rapid, label-free detection of analytes....
In 2015, the first commercial nanopore DNA sequencing device was introduced by Oxford Nanopore Technologies. Based on a synthetically engineered transmembrane protein, nanopore sequencing allows long DNA strands to be channelled through the central lumen
Single-stranded DNA hairpins produced identical ionic current blockades in pores made from monomers and subunit dimers demonstrating that M1-M1 pores are suitable for DNA sequencing. This study provides the proof of principle that production of single-chain MspA pores in M. smegmatis is feasible ...
Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and long read-length DNA sequencing applications. The analysis and sequencing of proteins, however, is complicated by their folded structure and non-uniform charge. Here we show that an electro-osmotic flow through Fra...