Nanopore sequencing works on the principle that as nucleic acid molecules pass through a nanopore channel in a membrane that separates two electrolyte-filled chambers, it disrupts the current and produces a characteristic electrical signal.The chamber where sequencing occurs is called the cis side, whi...
Scott Hickey, Director of Genomic Applications at Oxford Nanopore, demonstrated how nanopore sequencing is being used forde novomonoclonal antibody discovery, analysing single-cell transcriptomes and the haplotype-resolved germline of the Ig locus. This comprehensive approach en...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions...
Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock proteindoi:10.1186/s13071-023-06073-9GREECECOLOMBIAHEAT shock proteinsLEISHMANIACUTANEOUS leishmaniasisVISCERAL leishmaniasis...
In the first step, the protein substrate was threaded into the nanopore by electrophoretic force (cis-to-trans). Then, ClpX was added to the cis solution to steadily pull the substrate protein back out of the pore (trans-to-cis). Nanopore sequencing allowed the examination of full-length,...
of controlling DNA movement through the nanopore (Akeson lab). These two elements were brought together for the first time in the summer of2010. By March2011, the chemistry had been developed and coupled to a basecalling algorithm, producing the first example of DNA sequencing with a nanopore....
Genotypicmethods基因型方法 NanoporesequencingasarapiddetectionandASTtest纳米孔测序作为快速检测和AST测试 ApplicationofNanoporesequencingforSalmonellaAST纳米孔测序在沙门氏菌AST中的应用 Antibioticresistanceisanurgentproblem抗生素耐药性是一个迫切的问题 Antibioticsusceptibilitytesting(AST)抗生素敏感性试验（AST）Antibiotic...
Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads. Here, we assess the performance of different methylation-callin...
Oxford Nanopore R&D teams have shown on MinION that the platform can be used to characterise amino acids in a short peptide sequence. This is an early step towards developing protein sequencing capabilities on the nanopore platform and further work will include further optimisation of chemistry to ...
1). The FASTQ file generated by the nanopore whole-genome sequencing consortium was used for the alignment process. The reported and the calculated depth of coverage was found to be ~ 30X. Then, it was down-sampled to 20X and 10X coverage only. The SV call set is used as a ...