Nanopore sequencing works on the principle that as nucleic acid molecules pass through a nanopore channel in a membrane that separates two electrolyte-filled chambers, it disrupts the current and produces a characteristic electrical signal.The chamber where sequencing occurs is called the cis side, whi...
产品解析|第13期:Oxford Nanopore Sequencing 第三代测序技术——测序界的“拍立得” 扬清芯片 让微流控研究变得更简单1 人赞同了该文章 测序技术从上世纪90年代初发展至今,取得了长足的进步,为“人类基因组计划“的发展提供了方法学基础。同时为人类在遗传疾病预测及临床检测、疾病诊断,高危疾病筛查及基因药物检测等...
Discover a new generation of molecular sensing technology which offers short to ultra-long native DNA and RNA reads.
During this online seminar, Phillip James, who is the Applications Manager for the UK at Oxford Nanopore Technologies, provides an in-depth introduction to sequencing the SARS-CoV-2 genome using the their protocol including advice for bes...
View our benchmarking data generated using nanopore sequencing Workflow: bacterial isolate sequencing To learn more about the protocol in this application note, you can download therapid end-to-end workflow for whole-genome sequencing of bacterial isolatesusing MinION Flow Cells on MinION or GridION ...
Oxford Nanopore Sequencing第三代测序系统的主要特点: 02 【芯片解析】 Oxford Nanopore 测序芯片整体解析,以MnION系列的Flow Cell为例: 一、芯片结构 二、使用过程 “芯片测序原理” 03 【小编点评】 一、产品优势 1.高通量: 纳米孔测序技术可同时对多个膜孔进行测序,每个膜孔可在极短的时间内完成一个 DNA 分...
Generate complete genome assemblies using long nanopore sequencing reads. Resolve structural variants, repeats, phasing, and base modifications.
Nanopore sequencing is the only sequencing technology to enable real-time analysis in fully scalable formats. From the pocket-sized MinION to the high-throughput, population-scale PromethION — scale nanopore sequencing to suit any experimental needs. ...
当前Nanopore测序下级数据仍然以Q7作为数据过滤的一个标准。PacBio三代测序平台由于有CCS校准模式(也有参数可以设置),一般来说HiFi数据个人觉得不用做质控,但对于现在的ONT数据个人建议做个质控查看一下数据质量,如果有需要可以做一下reads过滤。相信随着ONT的测序准确度越来越高以及Duplex测序方式,今后也可能弱化质控过程...
The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use of these reagents. 产品类别: Next Generation Sequencing Library Preparation Products 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 E7180S -20 NEBNext® FFP...