不同于前两代测序技术,第三代纳米孔测序技术无需PCR扩增,利用纳米孔特殊结构实现单条DNA/RNA分子的实时测序,从而克服了第二代测序技术(NGS)中存在的PCR扩增误差、测序读长短、DNA/RNA甲基化修饰的问题,并广泛应用于基因组测序、突变鉴定等多个研究领域。 01 【产品简介】 Oxford Nanopore Sequencing第三代测序系统...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Oxford Nanopore Technologies, initially conceptualized in the laboratories of David Deamer, George Church, and Hagan Bayley during the 1980s [45,46], has undergone maturity through the optimization of nanopores and motor proteins, multiple times of sequencing, and the development of novel base-calling...
The Loman, Quick and Simpson (LQS) pipeline [17] presents an excellent example on how a high quality assembly could be produced from nanopore sequencing data by following the steps of preassembly data processing, overlap detection, consensus generation, and finally contig base polishing using the ...
Oxford Nanopore—— 打印本文 小 中 大 袖珍型、便携式生物分析设备 多达512 个纳米孔通道 样本制备只需10 分钟,简单快捷 实时分析,工作流程快速高效 适用于 DNA 或 RNA 直接测序 MinION: 测序成本在1000 美元 无需等待的即时测序 能在实验室外进行实地测序 达到每48 小时10-20 Gb数据量 包含多个测序装置,...
Whole genome sequencing is an essential cornerstone of pathogen surveillance and outbreak detection. Established sequencing technologies are currently being challenged by Oxford Nanopore Technologies (ONT), which offers an accessible and cost-effective alternative enabling gap-free assemblies of chromosomes and...
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Reveal more complex biology in a single assay with nanopore sequencing. Perform comprehensive genomic, epigenomic (e.g. methylation), and transcriptomic analysis with any-length reads using streamlined workflows on a single platform.
Gordon Sanghera, PhD, labeled 2025 during his presentation at the recent 43rd Annual J.P. Morgan Healthcare Conference. The designation was Sanghera’s way of emphasizing Oxford Nanopore’s commitment over the next five years to combine proteomics with multi-omics in its menu of sequencing ...
Oxford Nanopore sequencing is a nanopore sequencing method developed by Oxford Nanopore Technologies (ONT) that identifies the sequence of nucleotides by passing individual molecules through nanoscale pores and measuring changes in the electrical current