最后使用Oxford NanoporePromethION平台进行测序。 数据下机后可使用Oxford Nanopore的wf-single-cell分析流程,对测序设备生成的原始纳米孔读长序列的输入文件(FASTQ)进行过滤和分析,并输出基因和异构体计数矩阵、UMAP细胞簇图等分析结果。 Oxford Nanopore单细胞工作流程 诺禾致源测试结果首发 使用Oxford Nanopore的单细胞...
最后使用Oxford Nanopore PromethION平台进行测序。 数据下机后可使用Oxford Nanopore的wf-single-cell分析流程,对测序设备生成的原始纳米孔读长序列的输入文件(FASTQ)进行过滤和分析,并输出基因和异构体计数矩阵、UMAP细胞簇图等分析结果。 Oxford Nanopore单细胞工作流程 Oxford Nanopore单细胞全长实测数据分享 通过对Oxfor...
总之,这些这些数据为了解牦牛适应性进化背后的遗传和细胞机制提供了重要信息。 文章题目:Long read genome assemblies complemented by single cell RNA-sequencing reveal genetic and cellular mechanisms underlying the adaptive evolution of yak 发表期刊:nature communications(IF=17.694) 发表时间:2022.09.06 主要研究结...
Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and u
Not if but when nanopore protein sequencing meets single-cell proteomics. Nat. Methods 20, 336–338 (2023). CAS PubMed Google Scholar Matzinger, M., Müller, E., Dürnberger, G., Pichler, P. & Mechtler, K. Robust and easy-to-use one-pot workflow for label-free single-cell ...
The nanopore community is stepping toward a new frontier of single-molecule protein sequencing. Here, we offer our opinions on the unique potential for this emerging technology, with a focus on single-cell proteomics, and some challenges that must be overcome to realize it.Keisuke Motone...
2. Porubsky, D. et al. Fully phased human genome assembly without parental data using single-cell strand sequencing and long reads. Nat. Biotechnol. 39, 302–308 (2021). 3. Guarracino, A., Heumos, S., Nahnsen,...
et al. Fully phased human genome assembly without parental data using single-cell strand sequencing and long reads. Nat. Biotechnol. 39, 302–308 (2021). 3. Guarracino, A., Heumos, S., Nahnsen, S., Prins, P. & Garrison, E. ODGI: understanding pangenome graphs. Bioinformatics 38, ...
Combining single-cell RNA sequencing with Nanopore long-read sequencing enables isoform-level analysis in single cells. However, due to the higher error rate in Nanopore reads, the demultiplexing of cell barcodes and Unique molecular Identifiers (UMIs) can be challenging. This tool enables the accur...
2. Porubsky, D. et al. Fully phased human genome assembly without parental data using single-cell strand sequencing and long reads. Nat. Biotechnol. 39, 302–308 (2021). 3. Guarracino, A., Heumos, S., Nahnsen, S., Prins, P. & Garrison, E. ODGI: understanding pangenome graphs. ...