Oxford Nanopore Sequencing第三代测序系统的主要特点: 02 【芯片解析】 Oxford Nanopore 测序芯片整体解析,以MnION系列的Flow Cell为例: 一、芯片结构 二、使用过程 0 “芯片测序原理” 03 【小编点评】 一、产品优势 1.高通量: 纳米孔测序技术可同时对多个膜孔进行测序,每个膜孔可在极短的时间内完成一个 DNA...
TraxION (in development) Sample-to-sequence portable device: Extract, prepare, sequence Find out more Recommended for you Related articles View all applicationsPromethION 2How to get started with nanopore sequencingHow to load a PromethION Flow Cell...
Oxford Nanopore Sequencing第三代测序系统的主要特点: 02 【芯片解析】 Oxford Nanopore 测序芯片整体解析,以MnION系列的Flow Cell为例: 一、芯片结构 二、使用过程 “芯片测序原理” 03 【小编点评】 一、产品优势 1.高通量: 纳米孔测序技术可同时对多个膜孔进行测序,每个膜孔可在极短的时间内完成一个 DNA 分...
Here we use real-time, on-device target enrichment ("adaptive") sequencing on a new type of low-cost nanopore flow cell as a highly multiplexed assay covering 1,147 antimicrobial resistance genes. Using this method, we detected four types of carbapenemase in a single isolate of Raoultella ...
2、测序总结文档(Sequencing_summary.txt),这个文件很重要,里面包含了每条序列的统计信息,质量值信息的,后面可以利用这个文件进行绘图。3、遥测信息文件(Sequence telemetry.js),包含测序过程中的温度/电压/纳米孔状态等信息4、 Guppy碱基识别软件日志(guppy_basecaller_1og-2019-._-。*.log) 利用guppy拆分条码数据 ...
5、共有序列的测序(consensus sequencing)方法 MinION测序数据目前只有92%的准确性。在低深度测序的情况下,不能够满足类似单体型(haplotype phasing)和人样品的SNV检测的要求。文章提到的解决问题的方法是rolling circle amplication,它的原理是将一个片段进行多次扩增,在一个DNA分子上生成多个拷贝,这样最终获得的共有序列...
但是,我们相信,这条道路通向的,一定是一个崭新的世界! Hello,未来! Hello,Next-generation Omics ! 参考文献 [1] MJain, S Koren, J Quick, et al. Nanopore sequencing and assembly of a human genome with ultra-long reads. bioRxiv.2017
Fully characterise human genomic, epigenomic, and transcriptomic variation with real-time nanopore sequencing. With nanopore technology, reveal critical insights for human genomics research — from developmental biology and rare disease genomics to comm
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Sequence your libraryon a flow cell and device that suits your needs Analyse your datafrom as soon as you start sequencing Recommended for you Related areas Nanopore sequencing accuracyData analysis resourcesHuman genomicsPopulation genomicsGenome assemblyView all applications...