at which point the run can be stopped. the flow cell wash kit provides a highly effective means of removing a library that has been loaded onto a flow cell. once the library has been removed, the flow cell can be re-used immediately, or stored for later usage. the washing procedure is...
, 999 . 00 what's in the box 1 x minion sequencing device min-101b 1 x control expansion kit exp-ctl001 1 x flow cell wash kit exp-wsh004 1 x license and warranty 60 months - mk1b slw60m-mk1b 2 x flow cells 1 x sequencing kit product information 4 fully released products from...
Rapid Barcoding Kit 96 V14 (SQK-RBK114.96), Flow Cell Wash Kit (EXP-WSH004), Flow Cell Wash Kit XL (EXP-WSH004-XL), MinION Flow Cell R10 (FLO-MIN114), MinION Flow Cell R9 (FLO-MIN106D), PromethION Flow Cell R10 (FLO-PRO114M), PromethION Flow Cell R9 (FLO-PRO002), P2 Solo...
All flow cells (MinION and Flongle) were provided by ONT. All probe sequences were custom designed. Barcoded probes and miRNAs were synthesized by Integrated DNA Technologies (Supplementary Tables1and3). Proteins were obtained from the following: thrombin (Invitrogen, catalogue no. RP-43100); B...
wash of libraries. Next, 50 fmol samples of the library were sequenced on either a MinION FLO-MIN106D R9.4.1 flow cell or PromethION FLO-PRO002 R9.4.1 flow cell, according to the manufacturer’s protocol. A sequencing depth of 40,000 reads per cell was aimed for, which for 500 ...
Flow Cell Flush (FCF)andFlow Cell Tether (FCT)These reagents are combined to make up the flow cell priming mix. The tether is used to bring the DNA strand to the membrane and to improve DNA capture by approximately 10,000-fold compared with capture without tether. ...
Use of a flow cell wash kit containing DNase I unblocks the pores, allowing reloading of further library aliquots over a 72-h period, increasing yield. The workflow we describe provides a novel solution to the need for a rapid, robust, scalable, cost-effective ORF15 screening protocol....
Another attractive property of nanopore sequencing is its ability to sequence in real-time. Users can terminate the run when their sequencing needs have been met, wash the flow cell and even recycle it for future use. We were thus interested to know if there was a “sweet-spot” for MOTU...
We performed a "flow cell-refuel" step after approximately 18-20 h of runtime by adding 75 µL of a 1:1 water-SQB-Buffer (SQB = Sequencing Buffer) mixture to the flow cell via the SpotON port. SQB-Buffer is part of the Oxford-Nanopore SQK-LSK109 Kit. Illumina sequencing was ...
Each time a run reached saturation; the flow cell was washed using ONT’s flow cell wash kit (EXP-WSH002). Flow cells 1 and 2 were loaded three times and twice, respectively. All data were merged before whole-genome de novo assembly. 4.5. ONT Sequence Analysis and De Novo Assembly ONT...