flow cell wash kit provides a highly effective means of removing a library that has been loaded onto a flow cell. once the library has been removed, the flow cell can be re-used immediately, or stored for later usage. the washing procedure is a simple two-step process and the kit ...
protocol, extra reagents will be required. compatibility the native barcoding kit 24 v14 can be used together with: kits native barcoding auxiliary kit v14 (exp-nba114) flow cell wash kit (exp-wsh004) flow cell priming kit (exp-flp004) flow cell priming kit xl (exp-flp004-xl) sequencing...
In terms of multiplexing samples on a flow cell, the protocol is optimised for native barcoding, and at the moment we have 24 barcodes available. We are working towards 96 native barcodes. You can multiplex up to 24 samples on all ...
containing an aperture with a diameter of 100 µm was used to separate two compartments of a flow cell. 5 µL of a solution of 5% hexane in pentane (v/v) was applied near the aperture of the Teflon membrane. After an evaporation period of 1 min, 400 µL buffer, ...
(Flongle) sequencing buffer. The volume for each sequencing experiment was determined by the flow cell used: 150 µl (MinION) or 30 µl (Flongle). After each experiment, the MinION flow cell was washed with the flow cell wash kit (ONT) according to the manufacturer’s protocol...
Another attractive property of nanopore sequencing is its ability to sequence in real-time. Users can terminate the run when their sequencing needs have been met, wash the flow cell and even recycle it for future use. We were thus interested to know if there was a “sweet-spot” for MOTU...
Flow Cell Flush (FCF)andFlow Cell Tether (FCT)These reagents are combined to make up the flow cell priming mix. The tether is used to bring the DNA strand to the membrane and to improve DNA capture by approximately 10,000-fold compared with capture without tether. ...
Use of a flow cell wash kit containing DNase I unblocks the pores, allowing reloading of further library aliquots over a 72-h period, increasing yield. The workflow we describe provides a novel solution to the need for a rapid, robust, scalable, cost-effective ORF15 screening protocol....
The WTA products from virus-positive sample 4.1 and virus-negative sample 1.1 were prepared for nanopore sequencing using ONT’s 1D Ligation Sequencing library preparation kit (SQK-LSK108), following the manufacturer’s protocol. The library was loaded onto an R9.4 flow cell. Two separate flow ...
3 From −5 to −20 mV HT blockade lifetimes were measured by applying a cyclic sweep voltage protocol consisting of 3 steps. In the first “capture” step, the applied potential was set to −60 mV for 2 seconds. In the second “release” step the applied potential was decreased to...