protein identificationnanoporestrypsinenzymatic degradationPresently, proteins are identified by cleaving them with proteases, measuring the mass to charge ratio of the fragments with a mass spectrometer, and matching the fragments to segments within known proteins in databases. We earlier demonstrated that ...
et al. Nanopore-based protein identification. J. Am. Chem. Soc. 144, 2716–2725 (2022). CAS PubMed Google Scholar Piguet, F. et al. Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore. Nat. Commun. 9, 966 (2018). PubMed ...
While current proteomics techniques suffer from limitations in sensitivity and/or throughput, nanopore technology has the potential to enable de novo protein identification through single-molecule sequencing. However, a significant barrier to achieving this goal is controlling protein/peptide translocation ...
chemical catalysis and biophysical characterization. We highlight the prospects of applying nanopores for single-protein analysis and sequencing, single-molecule covalent chemistry, clinical sensing applications for single-molecule liquid biopsy, and the use of synthetic biomimetic nanopores as experimental mod...
Finally, CPC/CNCI/CPAT/Pfam were used to demonstrate the protein-coding genes from the non-coding genes. DETs and DEGs analysis Genes are the basic units that control the transmission of genetic information in an organism, while transcripts are the products of gene expression. Since several ...
The circum-basmati group of cultivated Asian rice (Oryza sativa) contains many iconic varieties and is widespread in the Indian subcontinent. Despite its economic and cultural importance, a high-quality reference genome is currently lacking, and the grou
RNA modifications contribute to RNA and protein diversity in eukaryotes and lead to amino acid substitutions, deletions, and changes in gene expression levels. Several methods have developed to profile RNA modifications, however, a less...
Several approaches to protein identification are being explored. Folded proteins have been sensed using solid-state42,43 and bio- logical44–46 nanopores. Properties such as protein volume, dipole and shape can be inferred by analysing the translocation dynam- ics of proteins through solid-state ...
through a nanopore (sometimes referred to herein as a “pore”) while its sequence is locally read, allows for long uninterrupted read-lengths at low copy number and its feasibility has recently been demonstrated by the introduction of a commercial sequencer based on protein nanopores (see, Jain...
exosomes are stable in the circulation and are found in all bodily fluids. They can hence be used as a powerful non-invasive diagnostic tool for many diseases including cancer. Third, exosomes represent their parental disease/tumor-specific RNA and protein profile, and their architecture protects ...