Oukhaled A, Pastoriza-Gallego M, Bacri L, Mathe J, Auvray L, Pelta J: Protein unfolding through nanopores. Protein Pept Lett 2014, 21:266-274.Oukhaled A, Pastoriza-Gallego M, Bacri L, Mathe J, Auvray L, Pelta J. Protein unfolding through nanopores. Protein Pept Lett. 2014; 9 :266...
The study demonstrated a co-translocational unfolding mechanism; first, the protein-tethered oligonucleotide is captured by the nanopore. Second, the oligonucleotide is pulled through the nanopore and the C-terminus of the protein locally unfolds because of the pulling force. Third, the remaining ...
Moreover, nanopores represent ultrasensitive sensors for revealing critical features of proteins, such as shape and size17, post-translational modifications18,19, enzymatic activity20,21, and mechanical unfolding22,23. In addition, nanopores are utilized to fingerprint proteins using enzymatic degradation...
Protein unfolding and translocation through pores occurs during trafficking between organelles, protein degradation and bacterial toxin delivery. In vivo, co-translocational unfolding can be affected by the end of the polypeptide that is threaded into the pore first. Recently, we have shown that co-...
Twenty individual amino acids can be distinguished by biological nanopores. Nanopores allow the discrimination of single-amino acid substitutions within proteins. Electro-osmotic flow allows uncharged and heterogeneously charged proteins to translocate through nanopores. ...
Paper Controlling the motion of DNA in a nanochannel with transversal alternating electric voltages
Oukhaled A, Bacri L, Pastoriza-Gallego M, Betton J-M and Pelta J 2012 Sensing proteins through nanopores fundamental to applications. ACS Chem. Biol. 7 1935–1949 Article CAS Google Scholar Perkins SJ 1986 Protein volumes and hydration effects. Eur. J. Biochem. 157 169–180 Article CAS ...
There are persistent challenges, though, and the authors are clear about that. Proteins differ in ease of unfolding, so there may not be universal conditions for translocation. Capture rate will also probably differ between proteins. Some PTMs are particularly compact (for example, methylation), ...
Herein, we report a dynamic monitoring approach based on nanopore single-molecule biosensor, which can detect the protein's folding and unfolding transitions by recording a nanopore ionic current. When gradually increasing the concentration of denaturant guanidine hydrochloride (GdmCl), sensitive ...
Characterization of protein unfolding with solid-state nanopores. In this work, we review the process of protein unfolding characterized by a solid-state nanopore based device. The occupied or excluded volume of a protein... Jiali,Li,Daniel,... - 《Protein & Peptide Letters》 被引量: 21发表...