Furthermore, we showed that the introduction of an aromatic residue in the sensing region of FraC (G13F-FraC containing a glycine to phenylalanine substitution at position 13) augmented the residence time of peptides inside the nanopore and their capture efficiency16. In this contribution, we ...
In this Review, we discuss recent advances in nanopore fabrication and sensing strategies that endow nanopores not only with sensitivity but also with selectivity and high throughput, and highlight some of the challenges that still need to be addressed....
Herein, we addressed these outstanding issues to the nanopore single-molecule biosensor, which provided an effective platform for protein denaturation in near-real physical environment, and particularly collected data based on individual difference instead of ensemble average. Nanopore sensing is a powerful...
Nanopores have attracted much interest for nucleic acid sequencing, chemical sensing, and protein folding at the single molecule level. The outer membrane protein OmpG from E. coli stands out because it forms nanopores from single polypeptide chains. This property affords one to separately engineer ...
Nanopore-based sequencing platforms are transforming the field of genomic science. This review (containing 116 references) highlights some recent progress on nanopore-based nucleic acid analysis and sequencing. These studies are classified into three categories, biological, solid-state, and hybrid nanopores...
Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores The simultaneous detection of a large number of different analytes is important in bionanotechnology research and in diagnostic applications. Nanopore sensing is an attractive method in this regard as the app...
The transport of polymers across nanoscale pores underpins many biological processes, such as the ejection of bacteriophage DNA into a host cell and the transfer of genes between bacteria. The movement of polymers into and out of confinement is also the basis for a wide range of sensing technolog...
These structural and biophysical features imply that FhuA ΔC/Δ4L has potential for applications in single-molecule stochastic sensing of nucleic acids and proteins [22], [23], [24], [25], [26], [27], but also in other biotechnological arenas [28]. We asked whether the ...
Peptide sequencing based on host–guest interaction-assisted nanopore sensing. Nat. Methods 21, 102–109 (2024). CAS PubMed Google Scholar Wang, K. et al. Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore. Nat. Methods 21, 92–101 (2024). ...
Design of the peptide nanopore provides insight into the application of nanopore sensing of DNA secondary structures and polypeptides, and also of hurdles encountered in nanopore technology. Our design strategy could also be integrated in the creation of molecular machines with use as part of a molec...