Furthermore, we showed that the introduction of an aromatic residue in the sensing region of FraC (G13F-FraC containing a glycine to phenylalanine substitution at position 13) augmented the residence time of peptides inside the nanopore and their capture efficiency16. In this contribution, we ...
In this Review, we discuss recent advances in nanopore fabrication and sensing strategies that endow nanopores not only with sensitivity but also with selectivity and high throughput, and highlight some of the challenges that still need to be addressed....
Herein, we addressed these outstanding issues to the nanopore single-molecule biosensor, which provided an effective platform for protein denaturation in near-real physical environment, and particularly collected data based on individual difference instead of ensemble average. Nanopore sensing is a powerful...
Nanopore Sensing of Protein Folding Single-molecule studies of protein folding hold keys to unveiling protein folding pathways and elusive intermediate folding states—attractive pharmaceutic... W Si,A Aksimentiev - 《Acs Nano》 被引量: 8发表: 2017年 ...
Nanopores have attracted much interest for nucleic acid sequencing, chemical sensing, and protein folding at the single molecule level. The outer membrane protein OmpG from E. coli stands out because it forms nanopores from single polypeptide chains. This property affords one to separately engineer ...
These structural and biophysical features imply that FhuA ΔC/Δ4L has potential for applications in single-molecule stochastic sensing of nucleic acids and proteins [22], [23], [24], [25], [26], [27], but also in other biotechnological arenas [28]. We asked whether the ...
nanoporepeptideelectroosmoticelectrophoreticproteincapture ElectroosmoticTrapAgainsttheElectrophoreticForceNearaProteinNanoporeRevealsPeptideDynamicsDuringCaptureandTranslocationAlinaAsandei,†,#IrinaSchiopu,†,#MauroChinappi,‡,#ChangHoSeo,§YoonkyungPark,*,∥andTudorLuchian*,⊥†DepartmentofInterdisciplinaryResearch...
In addition, the typical and updated applications of SSNP including DNA, protein and virus are briefly discussed. Finally, this article offers the context needed to comprehend current research trends and promote molecular sensing through synthetic nanopores....
Peptide sequencing based on host–guest interaction-assisted nanopore sensing. Nat. Methods 21, 102–109 (2024). CAS PubMed Google Scholar Wang, K. et al. Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore. Nat. Methods 21, 92–101 (2024). ...
This has been most notably achieved using an atomic force microscope in which proteins have been stretched in order to characterize folding forces6,15. In terms of high-throughput analysis of molecules, however, nanopores have several unique attributes when it comes to single molecule sensing. One...