single guide RNA 单导核酸
求翻译:single guide RNA是什么意思?待解决 悬赏分:1 - 离问题结束还有 single guide RNA问题补充:匿名 2013-05-23 12:21:38 单导的RNA 匿名 2013-05-23 12:23:18 一个指南RNA 匿名 2013-05-23 12:24:58 唯一指南RNA 匿名 2013-05-23 12:26:38 单指导 RNA 匿名 2013-05-23 12:2...
单向导RNA (sgRNA)是CRISPR/Cas9技术中用于基因编辑的关键要素,大小通常在100~150个碱基之间。本应用纪要表明,可以使用在Waters Protein-Pak Hi Res Q色谱柱上开发的优化阴离子交换方法,通过比较Low Range ssRNA Ladder(50-500个碱基)估算多个sgRNA的大小。此外,可以使用...
Optional: DNase treatment to remove DNA template. Typical RNA synthesis reactions using the HiScribe T7 Quick High Yield RNA Synthesis Kit can generate large amounts of RNA, at concentrations up to 10 mg/ml. As a result, the reaction mixture can be quite viscous. It is easier to perform D...
Synthetic single guide RNA (sgRNA) is sgRNA that is chemically synthesized using the latest technological advancements. Utilizing synthetic sgRNA is a simple and cost-effective way to improve your CRISPR editing, as it offers higher editing efficiency, lower cytotoxicity, and higher lot-to-lot stabil...
This tool uses best practices and the latest computational tools to deliver the optimal single guide RNA (sgRNA) sequence for every gene in the human and mouse genomes.
A key prerequisite for the success of CRISPR/Cas9 is its capacity to distinguish between single guide RNAs (sgRNAs) on target and homologous off-target sites. Thus, optimized design of sgRNAs by maximizing their on-target activity and minimizing their potential off-target mutations are crucial ...
A key prerequisite for the success of CRISPR/Cas9 is its capacity to distinguish between single guide RNAs (sgRNAs) on target and homologous off-target sites. Thus, optimized design of sgRNAs by maximizing their on-target activity and minimizing their potential off-target mutations are crucial ...
他们把他拿来做screen。对于我们刚刚一股脑转染的那一盘细胞,如果我们用来做Single Cell RNA-seq,我们就知道了每一个细胞的transcriptom,和control组比较,就知道了每个细胞的transcriptom发生的改变。 那如何知道我们对每个细胞施加的干预是什么呢?每个细胞里转进了几个guide RNA,每个guide是什么呢?很简单,给每一个gui...