sgRNA (single-guide RNA) is a fusion of crRNA and tracrRNA used in the CRISPR-Cas9 system, while gRNA (guide RNA) is a general term referring to RNAs that guide the binding of proteins.
利用CRISPR/Cas9技术构建敲除MEIS2基因的HEK293T人胚肾细胞系 CRISPR/Cas9系统作为一种新型的基因组编辑技术,利用人工设计的向导RNA(singleguide RNA,sg RNA)介导外源表达的Cas9蛋白与基因组靶点特异性结合以实现对基因组DNA的特... 卢利莎,白杨,刘鑫,... - 《中国细胞生物学学报》 被引量: 12发表: 2015年 ...
The RNA guide is a single strand that is complimentary to a certain target gene, for example, one that is making sickle cells. The Cas9 enzyme uses this RNA guide to “read” the host’s DNA like a map, to find the target gene. When a match happens, this triggers the enzyme’s in...
Molecule DNA-RNA Visualization This Amira Software module visualizes the typical ladder structure of double-stranded DNA or single-stranded RNA structures. Each strand consists of multiple nucleotides with four different base types (Adenin, Cytosine, Guanine, and Thymine for DNA...
The term “CRISPR library” is often used interchangeably with terms such as “CRISPR guide RNA library” or “sgRNA library.” Traditionally, this “RNA library” is really the batch of lentiviruses produced from the pool of oligos. Considering this, such a library is not actually free RNA,...
Guide RNA: a piece of RNA (a genetic cousin of DNA) that locates the targeted gene. This is engineered in a lab. CRISPR-associated protein 9 (Cas9): the “scissors” that snip the undesired DNA out DNA: the desired piece of DNA that is inserted after the break ...
MicroRNAs (miRNA) are single-stranded short RNA molecules that regulate gene expression by either degradation or translational repression of mRNA. Although... ST Lee,K Chu,KH Jung,... - Stroke; a journal of cerebral circulation 被引量: 313发表: 2010年 ...
DownloadThe CRISPR Basics Handbookthat covers applications ofCRISPR technologyfromguide RNAdesign to data analysis. The latestCRISPR abbreviations and definitionsin a single list! Design custom solutions for CRISPR genome editing. References: Anders C, Niewoehner O, et al. (2014)Structural basis of PA...
Guide to analyzing RNA-seq data What does the data look likeYoung, Matthew D
RNA is a critical component of every single living cell in the universe. Without it, life as we know it could not exist. There are three types of RNA, each with a unique function. mRNA is used to produce proteins from genes. rRNA, along with protein, forms the ribosome, which translate...