ArciTect™ sgRNAis a custom single guide RNA (sgRNA) for CRISPR-Cas9 genome editing. ArciTect™ sgRNA contains a user-specified 19- to 21-base sequence complementary to the target genomic location. ArciTect™ sgRNA must be designed directly upstream of a protospacer adjacent motif (PAM) s...
So designing precise single-guide RNAs (sgRNAs) to minimize off-target effects is critical for the success of gene editing without undesired results. Methods: Hence, in this article, three different online tools are used to design sgRNA to target the prolactin (PRL) gene to kn...
DeepCRISPR: optimized CRISPR guide RNA design by deep learning Genome Biol, 19 (1) (2018), 10.1186/s13059-018-1459-4 Google Scholar [13] L.i. Xue, B. Tang, W. Chen, J. Luo Prediction of CRISPR sgRNA activity using a deep convolutional neural network J Chem Inf Model, 59 (1) (...
A tool for genome-wide design of CRISPR guide RNAs reduces off-target effects and facilitates targeting of the non-coding genome. We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding gen
Already have your sgRNA design? Knockout HDR Knock-in Enter your sequence Looking for guides for other editing systems? Prime editing guide RNA (pegRNA) Synthesis Service 110 to 231 nt- the longest synthetic guides from any supplier- with editing efficiency over 60% ...
single guide RNA的中文翻译 single guide RNA 单导核酸
研究目的:本实验通过运用CRISPR/Cas9技术构建S100A8基因敲除的低分化鼻咽癌细胞CNE2细胞系,研究S100A8基因对鼻咽癌细胞CNE2的增殖以及侵袭迁移的影响。研究内容:研究... 孟盈 - 广西医科大学 被引量: 0发表: 0年 Construction of S100A8 Knockout CNE2 Cell Line by CRISPR-Cas9 Objective To construct the CNE2...
Recently, we achieved co-expression of Cas9 enzymes and the associated guide RNAs inE. colito prepare self-assembling Cas9 RNPs15. To purify these Cas9 RNPs, we harnessed a first Ni-NTA affinity purification and a following gel filtration step, resulting in a yield of ~10 mg Cas9 RNPs fr...
It mainly includes four parts: (1) introducing a guideRNA (gRNA) vector so that gRNA can be directly detected in single-cell transcriptome data; (2) high-throughput analysis of scRNA-seq; (3) a computational pipeline for assignment of single-cell to gRNAs; (4) analysis of single cell ...
One study developed a linear-tracking system (ClonMapper), which utilized DNA barcoding in conjunction with CROP-seq [122], which is an expression vector for single-guide RNA (sgRNA) capable of expressing and capturing sgRNA barcodes in scRNA-seq [123]. This innovative approach enabled direct ...