How does our gRNA design tool work? The IDT Custom Alt-R™ CRISPR-Cas9 guide RNA design tool can be used to help you design gRNA sequences for use with Cas9 with high on-target and low off-target activity in human, mouse, rat, zebrafish, and C. elegans for your specific research ...
Chapter 1: How to Design Guide RNA for CRISPRChapter 2: How to Use Synthego’s CRISPR Design ToolChapter 3: What is sgRNA? Step 2: Edit DNA Precisely with CRISPR Once the sgRNA is designed and synthesized, you can precisely edit the desired DNA sequence in any genome... right after you...
How to Design a good RNA-Seq experiment in an interdisciplinary context ?doi:10.7490/F1000RESEARCH.1096840.1Julie AubertAnne DelafoyeCyprien GuérinChristelle Hennequet-AntierFrédérique HilliouFabrice LegeaiDelphine LabourdetteNathalie MarsaudBrigitte Schaeffer...
1). Here, we use the biological model system of macrophage activation as a framework to demonstrate how we approach the study of lncRNA biology. We provide a step-by-step guide to consider when studying lncRNAs. Furthermore, we discuss the challenges, as well as the emergence of new ...
Somewhere along the line, I decided I was going to teach myself graphic design. I started off using PicMonkey (which is brilliant but pricey), but they started charging for their free tier, so I moved over to this shiny new platform called Canva. I’ve been using Canva for 7 years ...
Alternatively, a CRISPR–Cas9-based approach was used55, and strains were transformed using a plasmid expressing Cas9, a guide RNA and double-stranded PCR repair template from the same plasmids, followed by removal of the Cas9 plasmid by 5-fluoroorotic acid selection. PP7 loops were introduced ...
Amplicon location: When analyzing gene expression, it is good practice to treat your RNA samples with RNase-free DNase I to remove any residual genomic DNA (gDNA) before quantification. Whenever possible, design your assays to span an exon-exon junction to reduce the possibility of gDNA detection...
Lastly, we show how we can use the RSEM simulator to help us design sequencing experiments. Please note that this tutorial cannot replace the RSEM manual. In order to make full use of RSEM, we still recommend you to go over the manual. Prerequisites You need a server with Linux/Mac OS....
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“We need a method that can be flexibly adapted to sample type or aim of the project,” says Rastislav Horos, chief technology officer at Hummingbird Diagnostics, a company using RNA-Seq to develop microRNA-based diagnostics to guide the safe and effective use of therapeutics. ...