Argonaute proteins are the central effectors of RNA-guided RNA silencing pathways in eukaryotes, playing crucial roles in gene repression and defense against viruses and transposons. Eukaryotic Argonautes are subdivided into two clades: AGOs generally facilitate miRNA- or siRNA-mediated silencing, while ...
RNA-guided genome editing |[agrave]| la carte Cell death and differentiation is a monthly research journal focused on the exciting field of programmed cell death and apoptosis. It provides a single acc... P Horvath,R Barrangou - 《Cell Research》 被引量: 27发表: 2013年 RNA-guided genome ...
RNA-guided recombinase enzymes have been discovered that herald a new chapter for genome editing — enabling the insertion, inversion or deletion of long DNA sequences at user-specified genome positions. By Connor J. Tou & Benjamin P. Kleinstiver ...
1.RNA-guided CRISPR-associated (Cas) endonuclease (e.g. class II endonuclease Cas9) 2.Sequence-specific guide RNA(e.g. sgRNA) There are several ways to introduce Cas endonucleases and sequence-specific guide RNA into cells (Fig. 1)however, delivery as ribonucleoprotein (RNP) complex (Fig. ...
系统标签: genome rna human guided 基因组 crispr RNA-GuidedHumanGenomeEngineeringviaCas9PrashantMali1,5,LuhanYang1,3,5,KevinM.Esvelt2,JohnAach1,MarcGuell1,JamesE.DiCarlo4,JulieE.Norville1,andGeorgeM.Church1,2,*1DepartmentofGenetics,HarvardMedicalSchool,Boston,MA02115,USA2WyssInstituteforBiologically...
RNA-Guided Human Genome Engineering via Cas9 Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Ca... P Mali,L Yang,KM Esvelt,... - 《Science》...
RNA-Guided Human Genome Engineering via Cas9 DOI: 10.1126/science.1232033 , 823 (2013);339 Science et al.Prashant Mali RNA-Guided Human Genome Engineering via Cas9 This copy is for your personal, non-commercial use only. clicking here.colleagues, clients, or customers by , you can order ...
Box H/ACA ribonucleoproteins (RNPs), each consisting of one unique guide RNA and 4 common core proteins, constitute a family of complex enzymes that catalyze, in an RNA-guided manner, the isomerization of uridines to pseudouridines (Ψs) in RNAs, a reaction known as pseudouridylation. Over...
CRISPR-Cas systems revolutionized the genome engineering field but need to induce double-strand breaks (DSBs) and may be difficult to deliver due to their large protein size. Tn7-like transposons such as CRISPR-associated transposons (CASTs) can be repurposed for RNA-guided DSB-free integration,...
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol. advance online publication (29 January 2013). Mali, P. et al. RNA-guided human genome engineering via Cas9. Science published online (3 January 2013). Download references Rights and permissions Reprints and ...