Single-cell sequencing provides analysis of thousands of individual cells within complex environments, as opposed to traditional bulk sequencing methods that only provide a representative average of all cells. With developments in microfluidics and molecular barcoding, NGS-based technologies have increasingly...
单细胞测序技术(single cell sequencing) 单细胞测序技术(single cell sequencing)是指在单个细胞水平上,对基因组、转录组、表观组进行高通量测序分析的一项新技术,它能够弥补传统高通量测序的局限性,揭示单个细胞的基因结构和基因表达状态,反映细胞间的异质性。2013年,单细胞测序技术被《Science》将其列为年度最值得...
These sequencing-based technologies are increasingly being targeted to individual cells, which will allow many new and longstanding questions to be addressed. For example, single-cell genomics will help to uncover cell lineage relationships; single-cell transcriptomics will supplant the coarse notion of ...
本月,美国安德森癌症中心的研究人员在Cell上发表”Multiclonal Invasion in Breast Tumors Identified by Topographic Single Cell Sequencing (doi:10.1016/ 肿瘤细胞往往存在着各不相同的遗传特征,这被称为肿瘤内异质性。它们独特的细胞构成使得治疗变得更加困难。此前的单细胞DNA测序方法已经打开了探索肿瘤细胞的大门,成...
单细胞RNA测序(scRNA-seq )是一个飞速发展的领域,该技术帮助科研人员从一堆细胞(bulk)水平精细到单细胞(single-cell)水平研究基因的表达状态,从而可以更加精细的刻画科研对象的分子状态(例如细胞图谱,表达特征等)。目前该领域涌现了一大批用于单细胞RNA测序分析的方法,随着越来越多方法的出现,如何选择和组合使用这些方...
2017年7月,奥地利研究人员在Nature Methods上发表“Pooled CRISPR screening with single-cell transcriptome readout (doi:10.1038/ nmeth.4227)”文章,创造性地将CRISPR-Cas9和单细胞RNA测序两种极有前景的基因组学领域结合在一起,发明了这种被称作CROP-seq的单细胞测序方法 (CRISPR droplet sequencing, CRISPR液滴测...
Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and transcriptomic methods, and examples of their applications. For the integration of multilayered data sets, such as the transcriptome data ...
要在基因-细胞 scRNA-seq 矩阵expr和附带数据框meta上使用默认参数运行 Augur（其中包含cell_type和label...
Single-cell RNA sequencing predominantly employs short-read sequencing to characterize cell types, states and dynamics; however, it is inadequate for comprehensive characterization of RNA isoforms. Long-read sequencing technologies enable single-cell RNA isoform detection but are hampered by lower throughpu...
2017年7月,奥地利研究人员在Nature Methods上发表“Pooled CRISPR screening with single-cell transcriptome readout (doi:10.1038/ nmeth.4227)”文章,创造性地将CRISPR-Cas9和单细胞RNA测序两种极有前景的基因组学领域结合在一起,发明了这种被称作CROP-seq的单细胞测序方法 (CRISPR droplet sequencing, CRISPR液滴测...