single-cell sequencingtechniqueneuroscienceUnderstanding the structure and function of life requires the study of its basic constituent unit鈥攃ells.Cell bio-function depends largely on the coordinated expression of different genes.Traditional sequencing methods(such as DNA sequencing,and bulk RNA-seq)are...
Single-cell sequencing provides analysis of thousands of individual cells within complex environments, as opposed to traditional bulk sequencing methods that only provide a representative average of all cells. With developments in microfluidics and molecular barcoding, NGS-based technologies have increasingly...
Reveal more biology with single-cell nanopore sequencing: perform gene- and isoform-level expression analysis and identify variants in a single experiment. Prepare samples for sequencing in just three hours and analyse data with the intuitive EPI2ME sing
This article provides a brief overview of the technical procedure and data processing involved in single-cell sequencing. It also reviews the current applications of single-cell sequencing in CRC research, aiming to enhance the understanding of intratumoral heterogeneity, CRC development, C...
Single-cell RNA Sequencing: Introduction, Methods, and Applications Introduction of single-cell RNA sequencing Single cell sequencinguses optimized next-generation sequencing (NGS) technology to check the sequence information of a single cell. There is a higher resolution of cell differences and a bette...
Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are transforming drug discovery and development. New opportunities are emerging in target identification owing to improved...
Therefore, single-cell sequencing technologies were widely used in the research of various tumors, and were of great significance for the development of new diagnostic and anti-tumor treatment methods (Fig. 2). Fig. 2 The clinical applications of single-cell sequencing in different fields, like ...
Precursor states of multiple myeloma (MM) and its native tumor microenvironment need in-depth molecular characterization to better stratify and treat patients at risk. Using single-cell RNA sequencing of bone marrow cells from precursor stages, monoclona
36, 37, 38 As a new and powerful tool for cancer research, single-cell sequencing technology also has many applications in CSC-related research fields. We review and summarize the related studies and present them in Table 2. It is extensively documented that BC metastasis is caused by cells ...
typically detecting thousands more transcripts per cell. Moreover, this method costs about €0.5–€1 per sequencing-ready cell library in 384-well plates with moderate cellular throughput. In this way, Smart-seq3 can count RNAs at allele and isoform resolution for large-scale applications across...