Ambion™ Single Cell-to-CT™ 试剂盒包含一个经验证的完整工作流程,适用于对含 1–10 个细胞的样品进行基因表达分析。每个试剂盒包含用于样品制备、逆转录、预扩增和 qPCR 的试剂,其已在仅5个步骤的简单工作流程中一起优化(如图所示)。主要产品特点:•单细胞实时荧光定量 RT-PCR 的预优化工作流程•单...
Single-cell RT-PCR gene expression profiling of acutely dissociated and immunocytochemically identified central neurons. J. Neurosci. Methods 136, 229-236 (2004).Volgin D V;Swan J;Kubin L.Single-cell RT-PCR gene expression profiling of acutely dissociated and immunocytochemically identified eentral ...
Durand, G.M. et al. (2006) Quantitative single-cell RT-PCR and Ca2+ imaging in brain slices. Pflugers Arch. 451, 716-726Durand G, Marandi N, Herberger S, Blum R, Konnerth A: Quantitative single-cell RT-PCR and Ca2+ imaging in brain slices. Pflugers Arch 2006, 451:716-726....
避免这一影响有两种处理,一是在进行逆转录之前先用DNAaseⅠ消化基因组DNA,另一种方法就是在设计PCR引物时尽量使引物结合序列跨越相邻外显子的结合处。 单细胞实时定量PCR技术(Single cell real-time quantitative PCR )由上海英拜生物科技有限公司 为您提供,如您想了解更多关于单细胞实时定量PCR技术(Single cell real...
方法:采用倍比稀释相对定量单细胞RT-PCR方法分离纯化人外周血中性粒细胞并提取总RNA,针对IL-8RβmRNA设计引物,进行RT-PCR确认IL-8Rβ在中性粒细胞中的基因表达并优化反应条件。 2) Single cell nested RT-PCR 单细胞巢式RT-PCR 3) in-cell reverse transcription poly-merase chain reaction(in-cellRT-PCR) ...
The protocol describes how to extract a single, living Purkinje cell for reverse transcription, divide the cDNAs into three equal samples and subject those to triplicate amplification of multiple targets by two rounds of PCR (first a multiplex PCR then a gene-specific nested PCR) and finally ...
避免这一影响有两种处理,一是在进行逆转录之前先用DNAaseⅠ消化基因组DNA,另一种方法就是在设计PCR引物时尽量使引物结合序列跨越相邻外显子的结合处。 单细胞实时定量PCR技术(Single cell real-time quantitative PCR )由上海英拜生物科技有限公司 为您提供,如您想了解更多关于单细胞实时定量PCR技术(Single cell real...
Pre-optimized workflow for real-time RT-PCR from single cells Maximum sensitivity for single-cell analysis Superior performance and reproducibility compared to alternative methods Complete kit convenience and easy-to-follow protocol Pre-optimized protocol ensures success and saves time Each workflow begins...
Single Cell PCR (Protocol provided by Carolyn Troeger) Cell picking c Axiovert 100/Zeiss, extended glass capillary/Drummond, Broomall and a micromanipulator/Zeiss Transfer cell into PCR tube containing 5 µl of 400 ng/µl Proteinase K/17 µM SDS (50 µl stock = 1 µl 20 mg/ml...
In this study we illustrate the use of a very sensitive protocol of semiquantitative single-cell RT-PCR that we have developed. This technique allows the simultaneous analysis of up to eight genes on a single cell. Here we show the experiments performed to validate this novel assay. As an ...