(1) suggested that RT-PCR could be performed on whole cell lysates without prior RNA isolation. These researchers developed a technique in which whole cells(generally less than 250) are lysed in a solution containing Recombinant RNasin(R) Ribonuclease Inhibitor (Cat.# N2511) byfreeze/thaw. The...
避免这一影响有两种处理,一是在进行逆转录之前先用DNAaseⅠ消化基因组DNA,另一种方法就是在设计PCR引物时尽量使引物结合序列跨越相邻外显子的结合处。 单细胞实时定量PCR技术(Single cell real-time quantitative PCR )由上海英拜生物科技有限公司 为您提供,如您想了解更多关于单细胞实时定量PCR技术(Single cell real...
避免这一影响有两种处理,一是在进行逆转录之前先用DNAaseⅠ消化基因组DNA,另一种方法就是在设计PCR引物时尽量使引物结合序列跨越相邻外显子的结合处。 单细胞实时定量PCR技术(Single cell real-time quantitative PCR )由上海英拜生物科技有限公司 为您提供,如您想了解更多关于单细胞实时定量PCR技术(Single cell real...
Ambion™ Single Cell-to-CT™ 试剂盒包含一个经验证的完整工作流程,适用于对含 1–10 个细胞的样品进行基因表达分析。每个试剂盒包含用于样品制备、逆转录、预扩增和 qPCR 的试剂,其已在仅5个步骤的简单工作流程中一起优化(如图所示)。主要产品特点:•单细胞实时荧光定量 RT-PCR 的预优化工作流程•单细...
(2005) Quantitative single-cell RT-PCR and Ca 2+ imaging in brain slices. Pflugers Arch 451 , 716–726.Durand G, Marandi N, Herberger S, Blum R, Konnerth A: Quantitative single-cell RT-PCR and Ca 2+ imaging in brain slices. Pflugers Arch 2006, 451: 716–726....
随后同时对每个单个细胞的TCRαβ转录本和DNA barcode进行RT-PCR扩增,细胞barcode可用于在单细胞水平将TCR序列与其同源抗原进行配对。Zhang等采用此方法同时筛选出超过150中癌新抗原和野生型多肽对,这说明可以利用TetTCR-seq高通量识别具有功能活性的新抗原特异性TCR。
et al. Allele-specific expression patterns of interleukin-2 and Pax-5 revealed by a sensitive single-cell RT-PCR analysis. Curr. Biol. 10, 789–792 (2000). CAS PubMed Google Scholar Esumi, S. et al. Monoallelic yet combinatorial expression of variable exons of the protocadherin-α gene...
RT-PCR was used to detect gene expression in situ in single selected cells of tomato callus aggregates. The cytoplasm from one cell was removed with a micropipette viewed under a light microscope and used directly for RT-PCR , followed by nested PCR. This method to remove cytosolic contents ...
Single-Cell RT-PCR Analysis of TRPC Channels Expressed in Rat Cholinergic, Dopaminergic, Noradrenergic, and Serotonergic Neurons in the BrainNot Availabledoi:10.1016/j.bpj.2008.12.1322Kawano, TakeharuJones, Nicole M.Floreani, Christina V.
In this study we illustrate the use of a very sensitive protocol of semiquantitative single-cell RT-PCR that we have developed. This technique allows the simultaneous analysis of up to eight genes on a single cell. Here we show the experiments performed to validate this novel assay. As an ...