Here, we highlight recent progress in our understanding of how cells prevent and correct merotelic kinetochore attachments.doi:10.1016/S0962-8924(97)84238-9ChrisChristodoulouElsevierTrends in Cell Biology
Nature Cell Biology volume 26, pages 194–206 (2024)Cite this article 14k Accesses 19 Citations 219 Altmetric Metrics details Abstract Mitochondrial DNA (mtDNA) encodes essential subunits of the oxidative phosphorylation system, but is also a major damage-associated molecular pattern (DAMP) that ...
We further selected Lnc00857 for knockdown and performed cell function experiments to decode the role of TRLS in LUAD cell function regulation. In PCR assay, Lnc00857 was successfully knocked down in A549 and Calu3 cells after siLnc00857 transfection (Additional file 4C). The CCK-8 and EdU ...
protocol does not require primers againstVgenes for targeted enrichment, in contrast to the previous multiplex PCR approach (Extended Data Fig.2b). They likely represent products of partial or failed recombination and we reasoned that they are still biologically meaningful, reflecting a cell’s histo...
Molecular single cell analyses provide insights into physiological and pathological processes. Here, in a stepwise approach, we first evaluate 19 protocols for single cell small RNA sequencing on MCF7 cells spiked with 1 pg of 1,006 miRNAs. Second, we
The principle purpose of the class II safety cabinet (laminar flow hoods, biology safety cabinets, BSCs) is the effective protection of personnel, the environment and the experiment [94, 95], for example, biological material during cell culture establishing, cells subculturing from micro‐organisms...
For the cell death analysis in Extended Data Fig. 7j, harvested cells were incubated with Annexin-V, APC conjugate (A35110) for 15 min at room temperature in binding buffer (10 mM Hepes, pH 7.4, 140 mM NaCl, 2.5 mM CaCl2), according to the manufacturer’s protocol. Cells ...
The single-cell expression profile of mES and drug treated cells reveal cell heterogeneity, demonstrating the enormous potential of Paired-seq for cell biology, developmental biology and precision medicine.Similar content being viewed by others MAPS-seq: magnetic bead-assisted parallel single-cell gene ...
This price includes RT and PCR amplification, as well as final library preparation (Fig. 2D). From here on, this protocol is referred to as SS3. Quality measures for single cell RNA-seq Common quality metrices applied in scRNA-seq are library size and nr. of genes detected per cell. ...
通过qRT-PCR,作者没有在未分离的骨髓细胞中检测到Adrb2和Adrb3的表达,但发现Adrb2和Adrb3在LepR+细胞和脂肪细胞中表达水平相似。缺乏这两种受体都不会显著损害造血再生;然而,缺乏Adrb2和Adrb3确实显著损害了造血再生。因此,β2和β3肾上腺素能受体均能促进造血再生,而β1肾上腺素能受体是不可缺少的。