Here, we highlight recent progress in our understanding of how cells prevent and correct merotelic kinetochore attachments.doi:10.1016/S0962-8924(97)84238-9ChrisChristodoulouElsevierTrends in Cell Biology
Nature Cell Biology volume 26, pages 1309–1321 (2024)Cite this article 20k Accesses 87 Altmetric Metrics details Abstract Transcription factors (TFs) control specificity and activity of gene transcription, but whether a relationship between these two features exists is unclear. Here we provide ...
2.4 Real-time quantitative PCR Total RNA was extracted using TRIzol reagent (Invitrogen, USA) following the manufacturer's protocol. Complementary DNA was synthesized using PrimeScript RT Master Mix, and real-time PCR was performed using SYBR Premix Ex Taq II (TaKaRa, Japan) at 95°C for 30 ...
For the cell death analysis in Extended Data Fig. 7j, harvested cells were incubated with Annexin-V, APC conjugate (A35110) for 15 min at room temperature in binding buffer (10 mM Hepes, pH 7.4, 140 mM NaCl, 2.5 mM CaCl2), according to the manufacturer’s protocol. Cells ...
After detachment and collection of the labelled cells, membrane proteins were isolated using the ProteoExtract Native Membrane Protein Extraction Kit (EMD Millipore) according to the manufacturer's protocol. For pulldown of biotinylated proteins, 50 mL of NeutrAvidin Agarose Beads (50% aqueous slurry;...
Objectives Increasing evidences demonstrate a close correlation between epithelial-to-mesenchymal transition (EMT) induction and cancer lipid metabolism. However, the molecular mechanisms have not b...
Following homogenization of the mouse cecum and colon contents using glass beads in a Precellys 24 homogenizer (Bertin Technologies, Montigny, France), bacterial DNA was extracted using a QIAamp DNA Stool Mini Kit (Qiagen) according to the manufacturer's protocol. DNA extracts were stored at –80...
2.5 Quantitative Real-time PCR (qRT-PCR) To quantitatively confirm the mRNA expression levels of NEAT1 in CRC cell lines and tissues, real-time PCR was performed. Total RNA was extracted from each clone using TRIzol according to the manufacturer's protocol. Reverse transcription was carried out...
The experimental strengths and the similarities between the cellular and molecular processes present in C elegans and other animals across evolutionary time (metabolism, organelle structure and function, gene regulation, protein biology, etc) have made C elegans an excellent organism with which to study...
Following the manufacturer’s protocol, cDNAs were synthesized from 1 µg of total RNA. Real-time PCR was performed using SYBR Green qPCR kit (Takara, Tokyo, Japan) on a thermal cycler (Mx3000P; Biorad, Hercules, CA, USA). The primer sequences for bovine and rabbit gene used were ...