000 cells) and a GEM-X 5' chip (20,000 cells), and then processed on a Chromium X instrument followed by library preparation, sequencing, and data analysis.The aggregated
000 cells) and a GEM-X 5' chip (20,000 cells), and then processed on a Chromium X instrument followed by library preparation, sequencing, and data analysis.The aggregated
根据所用文库的制备方法,RNA序列((also referred to as reads or tags)将从转录本(10X Genomics, CEL-seq2, Drop-seq, inDrops)或全长转录本(Smart-seq)的3'端(或5'端)中衍生。 Image credit: Papalexi E and Satija R. Single-cell RNA sequencing to explore immune cell heterogeneity, Nature Reviews...
Single-cell cDNA, library preparation, and 3′-end single-cell RNA-sequencing were performed by Novogene (Beijing, China). For experiments using the 10× Genomics platform, the Chromium Single Cell 3′ Library & Gel Bead Kit v2 (PN-120237), Chromium Single Cell 3′ Chip kit v2 (PN-12023...
The ddSEQ 3’ RNA-Seq Library Prep Kit allows researchers to profile gene expression with single-cell precision. 单细胞测序用ddSEQ分离器 ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell chromatin accessibility studies. The system includes the isolator, test...
Comparative analysis for scRNA-seq library preparation Common steps required for the generation of scRNA-seq libraries include cell lysis, reverse transcription into first-strand cDNA, second-strand synthesis, and cDNA amplification. In general, cells are lysed in a hypotonic buffer, and poly(A)+ ...
Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible for cell reprogramming during somatic embryogenesis are still largely unclear. Recent advances in single-cell technol
(Thermo Fisher Scientific®#4456740). The NEBNext Single Cell/Low Input RNA Library Prep Kit, or the SMART-Seq v4 Ultra®Low Input RNA Kit for Sequencing (Clontech®# 634891) plus the Nextera XT DNA Library Prep Kit (Illumina #FC-131-1096) were used. Libraries were sequenced on ...
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has advanced our understanding of the pathogenesis of disease and provided valuable insights into new diagnostic and therapeutic strategies. With the expansion of capacity f
A common feature of single-cell RNA-seq (scRNA-seq) data is that the number of cells in a cell cluster may vary widely, ranging from a few dozen to several thousand. It is not clear whether scRNA-seq data from a small number of cells allow robust identif