Sequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext Single Cell/Low Input RNA Library Prep Kit, or the SMART-Seq v4 Ultra®Low Input RNA Kit for Sequencing (Clontech®# 634891) plus the Nextera XT DNA Library Prep Kit (Illumina®#FC-131-1096)...
In recent years, the combination of CRISPR screening with single-cell RNA sequencing (scRNA-seq) has enabled deep phenotyping of genetic perturbations at scale [53,54,55], providing an unprecedented opportunity to disentangle genome regulation. Specifically, high-throughput single-cell CRISPR-...
Numbat does not require paired DNA or genotype data and operates solely on the donor scRNA-seq data (for example, 10x Cell Ranger output). For details of the method, please checkout our paper: User Guide For a complete guide, please seeNumbat User Guide. ...
英文名称: Single Cell WTA Kit 纯度: 99 % 产地: 中国/上海 品牌: 泽叶生物 包装规格: 24 次 货号: ZY140450 别名: 单细胞全转录组扩增试剂盒 单细胞全转录组扩增试剂盒(Single Cell WTA Kit)提供了更简单、更快速和更高质量的方法,包括质粒提取、胶回收、PCR产物纯化、基因组提取、RNA分离纯化...
Accurate repair of DNA damage is critical for maintenance of genomic integrity and cellular viability. Because damage occurs non-uniformly across the genome, single-cell resolution is required for proper interrogation, but sensitive detection has remaine
Illumina ScriptSeq Complete Kit (H/M/R) 24 reactions BHMR1224 停产,替代货号20020596、20020597Illumina Illumina HiSeq 3000 Integration (per instrument per year) Hosted 20006532 30000Illumina Bio-Rad? ddSEQ? Single-Cell Isolator BIO-RAD 12004336 1200000Illumina ABI 7900HT Fast Real-Time PCR System ...
Here, using a combination of single-cell multiomics, spatial omics, massively parallel reporter assays and deep learning, we mapped enhancer-gene regulatory networks across mouse liver cell types. We found that zonation affects gene expression and chromatin accessibility in hepatocytes, among other ...
Colours in the diagram of the root tip indicate the corresponding cell type. c Enrichment of accessibility of proximal regulatory elements for marker genes as identified by single-cell RNA-seq. Dot diameter, proportion of cluster cells with proximal regulatory elements for a given gene; colour, ...
For this User guide I am using a public data set of PBMC single cell RNAseq data (https://doi.org/10.1101/123810). Gene and Cell selection A key feature of this app is the ability to change the cells and genes that are being used for the rest of the app. Note: The calculation ...
超详细单细胞悬液制备解决方案 PPT是给大家一个直观的认识: 细胞消化 细胞活性 细胞大小 杂细胞去除 试剂与仪器选择 url IUPUI Center for Medical Genomics, 10X scientists, and Miltenyi hosted a workshop on Cell Preparation for Single-Cell RNA-seq Experiments...