Sequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext Single Cell/Low Input RNA Library Prep Kit, or the SMART-Seq v4 Ultra®Low Input RNA Kit for Sequencing (Clontech®# 634891) plus the Nextera XT DNA Library Prep Kit (Illumina®#FC-131-1096)...
Hieff NGS® Single Cell/Low Input RNA Library Prep Kit是兼容Illumina®和MGI®双平台,可以对1~1000个细胞或者10 pg~10 ng的总RNA进行测序文库构建的试剂盒。本试剂盒可以进行全长cDNA的合成,完成全长cDNA的富集,然后对获得的全长cDNA扩增产物进行酶切法文库构建,与传统的建库方法比较,本品采用高质量的片...
In recent years, the combination of CRISPR screening with single-cell RNA sequencing (scRNA-seq) has enabled deep phenotyping of genetic perturbations at scale [53,54,55], providing an unprecedented opportunity to disentangle genome regulation. Specifically, high-throughput single-cell CRISPR-...
单细胞全转录组扩增试剂盒(Single Cell WTA Kit) 产品及特点 本产品是用于对从单个细胞中得到的RNA或mRNA 进行全转录组扩增的试剂盒,本产品能够能从极微量的单细胞样本均一地扩增全部的转录物,以非常有限或甚至可忽略地偏差扩增,准确呈递单细胞的转录模式。扩增的cDNA非常适用于二代测序(RNA-Seq)、基因表达芯片或...
Length Biases in Single-Cell RNA Sequencing of pre-mRNAdoi:10.1101/2021.07.30.454514Gennady GorinLior Pachter
Numbat does not require paired DNA or genotype data and operates solely on the donor scRNA-seq data (for example, 10x Cell Ranger output). For details of the method, please checkout our paper: User Guide For a complete guide, please seeNumbat User Guide. ...
For most downstream analyses, we focused on the six most common main cell types (Fig. 2a,b). Fig. 2: Summary of single-nucleus RNA-seq and ATAC-seq data from rat amygdala. a, UMAP plot of snRNA-seq data from rat amygdala. Data are combined across 19 samples, with high, low and ...
To improve the resolution, we also performed four 10x single-nucleus RNA-seq (snRNA-seq) and two 10x single-nucleus ATAC–seq (snATAC–seq) experiments, which provided an additional 16,900 single-cell transcriptomes and 9,702 single-cell ATAC profiles, respectively (Fig. 1a). From the ...
Recently, single-cell RNA sequencing (scRNA-seq) has been applied to root cells of Arabidopsis and rice and revealed the transcriptional programmes of major cell types, as well as the heterogeneity within a given population [1,2,3,4,5,6,7,8,9]. However, the mechanisms underlying these pro...
德国QIAGEN,货号:150343,REPLI-g Single Cell Kit (24) 价格电议 起批量≥1 盒 最小起订1盒 供货总量66盒 发货地址广东省深圳市 建议售价￥0/盒 更新日期2024年12月20日 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP9年 深圳子科生物科技有限公司 ...