Frequently asked questions around next-generation sequencing and single-cell RNA-seq sample preparation and order processing.
Single-cell RNA libraries construction and sequencing After each high viability (>90%) single-cell suspension of fish immune organ was prepared, the single-cell RNA-seq library was constructed immediately. For library construction, we used the DNBelab C Series Single Cell RNA Library Preparation Kit...
For a long time, it was assumed that fibroblasts were a uniform population of spindle-shaped cells. However, emerging evidence indicates that fibroblasts are actually a morphologically and functionally heterogeneous cell population3,7,10,11,12. The development of single-cell RNA-sequencing (scRNA-s...
Single-cell RNA sequencing (scRNA-seq) data from complex human tissues have prevalent blood cell contamination during the sample preparation process. They may also comprise cells of different genetic makeups. We propose a new computational framework, Originator, which deciphers single cells by genetic...
Figure 1. scRNA-seq reveals cellular heterogeneity that is masked by traditional bulk RNA-seq methods. Know what you are working with Sample preparation protocols will differ for every sample type, but whichever protocol is utilized, steps should be taken to ensure that cells loaded into the syst...
Experiment-related workflow of scRNA-seq techniques Advances of individual-cell RNA sequencing in carcinoma Immune cells Non-immune cells Combination of scRNA-seq and other methods Use of individual-cell RNA sequencing inside different fields Conclusions and perspective Author contributions Declaration of ...
Thanks to innovative sample-preparation and sequencing technologies, gene expression in individual cells can now be measured for thousands of cells in a single experiment. Since its introduction, single-cell RNA sequencing (scRNA-seq) approaches have revolutionized the genomics field as they created unp...
(B) Once a neoantigen-reactive TIL population was identified, this polyclonal TIL population was co-cultured with neoantigen-transfected/pulsed antigen-presenting cells (APCs) for 4 hr, and it was subjected to single-cell RNA-seq sample preparation and sequencing, followed by bioinformatics analysis...
While RNA-seq provides gene expression data from one entire sample in bulk, single-cell analysis allows for a better characterization of gene expression associated to specific cell types. Single-cell RNA-seq (scRNA-seq) is a reliable technique to unravel transcriptomic features of the tissues of ...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughp...