Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription-PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would result in an ...
Using the whole-genome shotgun method as the primary approach to sequencing large genomes, notably the human genome, Celera has demonstrated the validity of this method, which is now used in genome-sequencing laboratories worldwide for all classes of organisms.Venter...
Population shifts from high proportions of lactobacilli to mixed species communities, as seen with bacterial vaginosis, have been linked to a twofold increased risk of PTB. Despite the increasing number of studies using next-generation sequencing technologies, primarily involving 16S rRNA-based approaches...
The completion of the sequencing of the mouse genome promises to help predict human genes with greater accuracy. While current ab initio gene prediction pr... Parra,G. - 《Genome Research》 被引量: 408发表: 2003年 Mouse segmental duplication and copy number variation content of mouse (4.94%)...
方式二:whole-genome shotgun sequencing:(数量小)。 (IHGSC):The International Human Genome Sequencing Consortium Sanger测序可以获得高质量数据,eg:测定的小鼠数据可以帮助理解小鼠家系,查看序列精度高。这种技术对未来的研究产生深远影响。 虽然造价高昂,但是sanger测序仍旧是得到高质量序列的主流方式。所以获得了大量...
Recently, metagenomic sequencing has revealed information about the complement of genes in the gut microbiota of two healthy individuals (Gill et al., 2006). Although this dataset did not represent the Human gut metaproteome NC Verberkmoes et al 180 entire GI microbiota, analysis of identified ...
Human Whole-Genome Shotgun Sequencing Large-scale sequencing of the human genome is now under way (Boguski et al. 1996; Marshall and Pennisi 1996). Although at the beginning of the Ge-nome Project, many doubted the scientific value of sequencing the entire human genome, thes... JL Weber,...
Durable coexistence of donor and recipient strains after fecal microbiota transplantation. Science 352, 586–589 (2016). Article CAS PubMed Google Scholar Kuczynski, J. et al. Direct sequencing of the human microbiome readily reveals community differences. Genome Biol. 11, 210 (2010). Article ...
shotgun sequencing has the full potential to draw up new dimensions in biodiversity research (Papadopoulou et al., 2015). The direct sequencing of genomic DNA from bulk or environmental samples allows the parallel acquisition of a large array of genetic information including multiple mitochondrial, pl...
amapping data. Repeat sequences were identified through sequence similarity at the [translate] aThe RH genotype was sequenced using shotgun sequencing of BACs and WGS in [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译...