In this study, we characterized the microbiome and for the first time, the composition of functional genes of healthy skin (HS), active (ADD) and inactive (IDD) lesion stages using a whole-genome shotgun approach. Metagenomic sequences were annotated using MG-RAST pipeline. Six phyla were ...
Shotgun metagenomic DNA sequencing does not rely on PCR amplification using gene-targeted primers; thus, it is a powerful technique in the field of molecular microbial ecology [8]. Because of the technical differences, amplicon sequencing and metagenomic shotgun sequencing may not give identical res...
which can confound cataloging of members and understanding of how communities function. High-throughput sequencing technologies and a suite of computational pipelines have been combined into shotgun metagenomics methods that have transformed microbiology. Still, computational approaches to overcome the challenge...
Although amplicon sequencing offers cost-effective and rapid profiling of microbial community structure and taxonomy [23], shotgun sequencing facilitates a more comprehensive assessment of the microbiome [21]. This approach not only yields more accurate taxonomic profiling, including the identification of ...
In this study, we characterized the microbiome and for the first time, the composition of functional genes of healthy skin (HS), active (ADD) and inactive (IDD) lesion stages using a whole-genome shotgun approach. Metagenomic sequences were annotated using MG-RAST pipeline. Six phyla were ...
Genome sequencing has opened up entirely new realms of possibility. Most obviously, it provides the basic infrastructure to detect genetic differences (mutations) which can help to un- derstand observed phenotypic differences (Genomics and SNPs). This sequence infrastructure also facili- tates the ...
“shotgun” method are it may under-represent some fragments, sequencing must be redundant to ensure the entire sequence was included in one or more subclones, and there is no way to identify specific fragments. But, as you will discover, the shotgun approach was adopted widely due to ...
GENOMICS: Shotgun Sequencing of the Human Genome The recent public announcement by TIGR and Perkin-Elmer that they intend to form a new company whose goal is to sequence the human genome four years faster and at lower cost than the government-backed Human Genome Project caught the imme... ...
Owing to all the effort devoted to explore entire genomes, we now have access to a multitude of completed genome sequences, which has recently opened up a new era of 'shotgun proteome sequencing'. The concept of shotgun proteomics basically consists of the multidimensional separation (MudPIT) ...
This approach should make a significant contribution to the early identification of important human genes, the deciphering of the draft human genome sequence currently being compiled, and the shotgun sequencing of the human transcriptome. 展开