Type I-C CRISPR-Cas system and 4 prophages were detected among the genome of 47S1. Several virulence genes were predicted, most of these genes are found in other pathogenic streptococci, mainly lmb, pavA, htrA/degP, eno, sagA, psaA and cpsI which play a significant role in colonizing, ...
方式一:多级策略:(IHGSC)工程量巨大(因为工程量巨大,所以急需的测序技术(新且造价低)),使用了BAC克隆同时高coverage(准确率高,数量大)。 方式二:whole-genome shotgun sequencing:(数量小)。 (IHGSC):The International Human Genome Sequencing Consortium Sanger测序可以获得高质量数据,eg:测定的小鼠数据可以帮助理...
Proteins, which are encoded by the genome, serve as key biological actors within cells and drive many integral biological processes in both healthy and diseased states. Shotgun proteomics, also known as "Bottom-up proteomics", is a widely used and mature technology for protein identification and ...
Introduction: Whole Genome Shotgun Sequencing (WGS) of the microbiota is a powerful technique that provides an unbiased look at the relative abundance of the gut microbiome, but the analysis of the large data sets requires a bioinformatician using software executed on high-powered servers. This ...
Using the strictest criteria currently available for the validation of ancient DNA sequences, whole-genome and whole-exome sequencing were generated from the mummy remains of an Italian nobleman died almost 700 years ago, Cangrande della Scala. While its genome sequencing could not yield sufficient...
The application of whole-genome shotgun sequencing to microbial communities represents a major development in metagenomics, the study of uncultured microbes via the tools of modern genomic analysis. In the past year, whole-genome shotgun sequencing projects of prokaryotic communities from an acid mine...
The present study applied whole genome shotgun sequencing (WGS) and single nucleotide variant (SNV) calling to characterize 279 cattle-shed E. coli O157:H7 strains isolated from a single feedlot located in southwestern region of the US. More than 4,000 SNVs were identified among the strains ...
we used these recent data based on the Illumina shotgun-sequencing technology and the present new sequence data obtained by sequencing Chicago and Hi-C proximity ligation libraries. The number and length of read pairs produced for Chicago library 1 was 223 million and 2 × 150 bp, respecti...
Two strategies introduced for whole genome sequencing,one is clone by clone method,the other is whole genome shotgun sequencing,for microbes which are very important to us,whole genome shotgun sequencing method is very convenient.In this article we discussed the library construction?long-to-short-...
微生物全基因组鸟枪法测序 Whole Microbial Genome Shotgun Sequencing 全基因组测序主要有二种策略,一种是分级鸟枪法测序,另一种是全基因组鸟枪法测序。微生物是一种十分重要的遗传资源,运用全基因组鸟枪法可以方便、快捷地完成其基因组的测序任务。本文对微生物全基因组鸟枪法测序中文库构建、插入片...更多>>全基...