The genome is sheared into a few thousand base pair long pieces and cloned into sequencing vectors. Each fragment end (~500 bp) is covered by the sequencing several times and then assembled into overlapping segments by end-sequences. In this way oriented, contiguous sequences (contigs or ...
The GSM (Rhinopithecus roxellana) whole-genome shotgun project has been deposited at the DNA Data Bank of Japan (DDBJ), the European Molecular Biology Laboratory (EMBL) and GenBank under the accession JABR00000000. The version described in this paper is the first version, JABR01000000. The Bio...
Optimized Multiplex PCR: Efficiently Closing a Whole-Genome Shotgun Sequencing Project A new method has been developed for rapidly closing a large number of gaps in a whole-genome shotgun sequencing project. The method employs multiplex PCR a... H Tettelin,D Radune,S Kasif,... - 《Genomics...
Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.
方式二:whole-genome shotgun sequencing:(数量小)。 (IHGSC):The International Human Genome Sequencing Consortium Sanger测序可以获得高质量数据,eg:测定的小鼠数据可以帮助理解小鼠家系,查看序列精度高。这种技术对未来的研究产生深远影响。 虽然造价高昂,但是sanger测序仍旧是得到高质量序列的主流方式。所以获得了大量...
Introduction: Whole Genome Shotgun Sequencing (WGS) of the microbiota is a powerful technique that provides an unbiased look at the relative abundance of the gut microbiome, but the analysis of the large data sets requires a bioinformatician using software executed on high-powered servers. This ...
The Whole Genome Shotgun project had been deposited at DDBJ/EMBL/GenBank under the accession JQOL00000000-JQSB00000000. The versions described in this paper were version JQOL01000000-JQSB01000000. References 1. Bharti, A. R. et al. Leptospirosis: a zoonotic disease of global importance. ...
Nucleotide sequence accession numbers. The Whole Genome Shotgun Sequencing projects of C. difficile isolates 103, 106 and 133 have been deposited into DDBJ/EMBL/GenBank under accession MBMH00000000, MBGC00000000 and MBGB00000000, respectively. References 1. Bartlett, J. G., Chang, T. W., ...
必应词典为您提供Whole-Genome-Shotgun-Sequencing的释义,网络释义: 全基因组鸟枪测序;全基因组鸟枪法测序;全基因组鸟枪测序技术;
Nucleotide sequence accession number. Reads and the Whole Genome Shotgun Sequencing project of C. freundii WCHCF65 have been deposited into DDBJ/EMBL/GenBank under the accession numbers SRR2153225 and LIDR01000000, respectively. The sequence of pKPC2_CF65 has been deposited into DDBJ/EMBL/...