This result indicates that the use of Normalase provides a faster, more efficient workflow for multiplex sequencing. In a shotgun metagenomic sequencing experiment, libraries were constructed using the xGen DNA Library EZ Kit and 1 ng of DNA derived from a mock metagenome DNA standard (ATCC, ...
Using the strictest criteria currently available for the validation of ancient DNA sequences, whole-genome and whole-exome sequencing were generated from the mummy remains of an Italian nobleman died almost 700 years ago, Cangrande della Scala. While its genome sequencing could not yield sufficient...
the genome of which was sequenced to produce the first reference assembly, including the mitochondrial genome7. To obtain the present new reference assembly, we used these recent data based on the Illumina shotgun-sequencing technology and the present new...
In this study, we performed whole metagenome shotgun sequencing of fecal samples to enhance the detection of rare bacterial species and increase genome sequence coverage. Additionally, we employed multiple bioinformatics approaches to investigate the potential targets of the microbiome as an indicator of...
We sequenced the genome of a male GSM using a whole-genome shotgun strategy. Cleaned-up data provided 146-fold average coverage across 3.05 Gb of assembled sequence (Supplementary Fig. 2 and Supplementary Tables 1 and 2), with N50 values of 25.5 kb and 1.55 Mb, respectively, for contigs ...
www.nature.com/scientificdata OPEN SUBJECT CATEGORIES » Genomics » Genome assembly algorithms » DNA sequencing » Experimental organisms Long-read, whole-genome shotgun sequence data for five model organisms Kristi E. Kim1, Paul Peluso1, Primo Babayan1, P. Jane Yeadon2, Charles Yu3, ...
Medvedev P, Brudno M. 2008. Ab initio whole genome shotgun assembly with mated short reads. In Proceedings of the 12th Annual International Conference on Research in Computational Molecular Biology (RECOMB). Lect Notes Comput Sci 4751: 50-64....
The bovine genome sequencing consortium sequenced a single inbred female Hereford cow and her sire using a combination of hierarchical sequencing and whole-genome shotgun sequencing [2]; the data have been assembled into two reference genomes, Btau and UMD [3, 4]. After the bovine reference ...
SMRT sequencing of theC.militarisgenome revealed many interchromosome translocations from the shotgun CM01 sequencing database (Fig.2a,c). As shown in Fig.2a,c, contig 3 of the HN strain genome is composed of scaffolds 1, 5 and 7 from the CM01 genome; contig 4 is composed of scaffolds...
Genome assembly and annotation We sequenced DNA from one male (ZZ) and one female (ZW)C. semilaevisseparately with the Illumina sequencing platform using a whole-genome shotgun approach with 212-fold coverage (Table 1,Supplementary Fig. 1andSupplementary Tables 1and2). We first assembled the ma...