the genome of which was sequenced to produce the first reference assembly, including the mitochondrial genome7. To obtain the present new reference assembly, we used these recent data based on the Illumina shotgun-sequencing technology and the present new...
Using the strictest criteria currently available for the validation of ancient DNA sequences, whole-genome and whole-exome sequencing were generated from the mummy remains of an Italian nobleman died almost 700 years ago, Cangrande della Scala. While its genome sequencing could not yield sufficient...
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We sequenced the genome of a male GSM using a whole-genome shotgun strategy. Cleaned-up data provided 146-fold average coverage across 3.05 Gb of assembled sequence (Supplementary Fig. 2 and Supplementary Tables 1 and 2), with N50 values of 25.5 kb and 1.55 Mb, respectively, for contigs ...
The bovine genome sequencing consortium sequenced a single inbred female Hereford cow and her sire using a combination of hierarchical sequencing and whole-genome shotgun sequencing [2]; the data have been assembled into two reference genomes, Btau and UMD [3, 4]. After the bovine reference ...
www.nature.com/scientificdata OPEN SUBJECT CATEGORIES » Genomics » Genome assembly algorithms » DNA sequencing » Experimental organisms Long-read, whole-genome shotgun sequence data for five model organisms Kristi E. Kim1, Paul Peluso1, Primo Babayan1, P. Jane Yeadon2, Charles Yu3, ...
prepared and sequenced shotgun-sequencing libraries using Illumina or BGI kits following manufacturer’s instructions in either paired-end 100-bp (case 4) or 150-bp read configuration. We targeted an average depth of 30× for WGS on parents’ and born children’s DNA. Actual mean coverage for...
Combining whole-genome shotgun sequencing and rRNA gene amplicon analyses to improve detection of microbe–microbe interaction networks in plant leaves Julian Regalado, Derek S. Lundberg, Oliver Deusch, Sonja Kersten, Talia Karasov, Karin Poersch, Gautam Shirsekar & Detlef Weigel The ISME...
SMRT sequencing of theC.militarisgenome revealed many interchromosome translocations from the shotgun CM01 sequencing database (Fig.2a,c). As shown in Fig.2a,c, contig 3 of the HN strain genome is composed of scaffolds 1, 5 and 7 from the CM01 genome; contig 4 is composed of scaffolds...
We used a whole-genome shotgun sequencing approach to generate 31 Gb of the raw sequence ofO. brachyanthausing the Illumina GA II platform (Supplementary Table S1). The genome was initially assembled using SOAPdenovo19, and the length of the sequence scaffold was further increased by integrati...