原文标题:Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin 原文链接:https://www.sciencedirect.com/science/article/abs/pii/S0092867420312538#! 来源:美国Broad Institute of MIT and Harvard 摘要 细胞分化和功能...
SHARE-seq, a new high-throughput, high-resolution multi-omics method described in Cell, measures chromatin accessibility and gene expression in the same cell and enables future potential gene expression (and therefore lineage choices) to be inferred from chromatin profiles....
https://github.com/masai1116/SHARE-seq-alignment/ After downloading all scripts, update the general configuration section in main script "Split_seq_example.sh": myPATH # where the SHARE-seq scripts are installed. e.g. myPATH='/mnt/users/Script/share-seq-github-v1/' pythohPATH # where p...
近日,来自美国Broad研究所和哈佛大学的研究团队在国际著名期刊《Cell》上发表题为 “Chromatin potential identified by shared single cell profiling of RNA and chromatin”的研究。 该研究开发了一种同时捕获RNA和染色质开放信号的方法:SHARE-seq。该技术能够实现在单细胞中同时高质量,高通量的检测基因表达和染色质可...
最近CELL杂志在线发表了可以同时检测单细胞染色质开放性和转录组的文章,这个可以在单细胞中同时检测染色质开放性和转录组的SHARE-seq技术全称为Simultaneous High-throughput ATAC and RNA Expression with sequencing(SHARE-seq).从名字就可以看出这是一个将单细胞转录组技术和ATAC-seq技术结合的产物,其中没有什么新的想...
细胞类型的鉴定(RNA和ATAC数据都可鉴定,且两者的相关性很好),并且像有些cell cycle基因高表达的细胞类型在RNA和ATAC上都可以找到。 SHARE-seq enables joint profiling of chromatin accessibility and gene expression in tissues 接着,同时表征两个模态,很重要的一件事是去看RNA和ATAC相关性。这里引出了DORCs,Peak...
Cell DIVE was used to expand this analysis to large, whole-tissue sections to improve the power of the study. They report fine details of how follicular histology is altered in early relapsers, and find that desmoplasia, extracellular matrix deposition, and stromal cell ...
An interactive R Shiny tool for visualizing single-cell RNAseq clustering results from common analysis pipelines. Its main goal is two-fold:A:to help select a biologically appropriate resolution or K from clustering results by assessing differential expression between the resulting clusters; andB:help...
1.Li, H. et al. Tranomic, epigenomic, and spatial metabolomic cell profiling redefines regional human kidney anatomy. Cell Metab 36, 1105-1125.e10 (2024). 2.Gu, Q. et al. Integrative profiling of untreated primary membranous nephropathy at the single-cell tranome level. Clinical Kidney Jour...
Single cell RNA-sequencing enabled by 10x Genomics assays allows for deep analysis of the transcriptome of individual cells, uncovering differences in heterogeneous cell populations that are often masked by traditional bulk methods. With ...