SHARE-seq是可扩展的平台,用于研究组织中不同细胞之间的调控通路。 内容 SHARE-Seq用于大规模联合分析染色质可及性和基因表达 为了创建染色质可及性和mRNA表达共谱分析方法,作者基于SPLiT-seq和Paired-seq方法开发了SHARE-seq,使用多轮杂交封闭来唯一且同...
最近CELL杂志在线发表了可以同时检测单细胞染色质开放性和转录组的文章,这个可以在单细胞中同时检测染色质开放性和转录组的SHARE-seq技术全称为Simultaneous High-throughput ATAC and RNA Expression with sequencing(SHARE-seq).从名字就可以看出这是一个将单细胞转录组技术和ATAC-seq技术结合的产物,其中没有什么新的想...
SHARE-seq provides an accurate co-measure of chromatin accessibility and gene expression 接着就是应用到具体的组织的研究了。细胞类型的鉴定(RNA和ATAC数据都可鉴定,且两者的相关性很好),并且像有些cell cycle基因高表达的细胞类型在RNA和ATAC上都可以找到。 SHARE-seq enables joint profiling of chromatin acces...
SHARE-seq, a new high-throughput, high-resolution multi-omics method described in Cell, measures chromatin accessibility and gene expression in the same cell and enables future potential gene expression (and therefore lineage choices) to be inferred from chromatin profiles....
ServingCellConfig ::= SEQUENCE { tdd-UL-DL-ConfigurationDedicated TDD-UL-DL-ConfigDedicated OPTIONAL,-- Cond TDD initialDownlinkBWP BWP-DownlinkDedicated OPTIONAL, -- Cond ServCellAdd downlinkBWP-ToReleaseList SEQUENCE (SIZE (1..maxNrofBWPs)) OF BWP-Id OPTIONAL, downlinkBWP-ToAddModList ...
研究人员选取饥饿再喂食和饥饿48h的小鼠肝脏进行染色质免疫沉淀测序(ChIP-seq)实验,证明组蛋白酰化可以在染色质上共同发生。 组蛋白的质谱分析证实H3K14pr和H3K14bu存在于小鼠肝脏中(图3a),H3K14pr和H3K14bu显示出与其他“活性”组蛋白标记相似的...
In June 2024, Bio-Rad launched the ddSEQ Single-Cell 3’ RNA-Seq Kit and Omnition v1.1 analysis software, enhancing single-cell gene expression research with efficient, cost-effective tools and workflows for diverse applications. Cell Counting Market Report Scope Report Attribute Details Market si...
Public SHARE-seq datasets are available atGEO. A set of fastq files for species mixing experiment can be downloadedhere. Cite us For more details, please refer toMa et al. Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin, Cell 2020...
近日,来自美国Broad研究所和哈佛大学的研究团队在国际著名期刊《Cell》上发表题为 “Chromatin potential identified by shared single cell profiling of RNA and chromatin”的研究。 该研究开发了一种同时捕获RNA和染色质开放信号的方法:SHARE-seq。该技术能够实现在单细胞中同时高质量,高通量的检测基因表达和染色质...
This is the process that happens when a LTE cell add NR Cell as a process of Dual Connectivity. Before you go into the details of this process, I would suggest you to read LTE-Interworking page and get some big picture first. There are two difference options (cases) of the RACH ...