In this study, we integrated published RNA-Seq datasets and established a landscape of REs during the development of mouse spermatogenesis.7530 editing sites among all types of male germ cells were found, which enrich on some regions of chromosome, including chromosome 17 and both ends of ...
performed plasmid construction, RNA-seq analysis, FACS and western blotting. W.L. assisted in confocal imaging. L.C. assisted with plasmid construction. Z.Z. performed chemical synthesis. X.S. assisted with mass spectrometry data analysis. M.N. assisted with marker protein selection. L.C. ...
All possible nucleotide at any single position was tested, with exceptions where A-to-G mutation was avoided in the +1 and −1 nt of NEIL1 because it potentially becomes indistinguishable with A-to-I editing in RNA-seq results. Combination mutations at two positions were also designed, ...
To run PREP, first fill file path of you rna-seq FASTQ file inconfg.yaml, then use: python PREP.py RE -i config.yaml For detailed information about usage, input and output files, test examples and data preparation please refer to thePREP User Manual ...
The experiments reported in the manuscript used three datasets built from theseFASTA files, extracted from nucleotide sequences encoding mitochondrial proteins from 21 plant species. In these files, 'E' nucleotides indicate C-to-U editing sites identified by using published RNAseq data, obtained from...
We here set out to obtain a systematic overview of iGluR splicing and editing in human brain based on RNA-Seq data. Using data from two large-scale transcriptome studies, we established a workflow for the de novo identification and quantification of alternative splice and editing events. We ...
RNA-seqgenomicsDravet syndrome (DS) is a disease that is primarily caused by the inactivation of the SCN1A-encoded voltage-gated sodium channel alpha subunit (Nav1.1). In this study, we constructed an SCN1A gene knockout model using CRISPR/Cas9 genome editing technology to deprive the Nav1.1...
Splicing and editing of ionotropic glutamate receptors: a comprehensive analysis based on human RNA-Seq data.doi:10.1007/S00018-021-03865-ZRobin HerbrechterNadine HubeRaoul BuchholzAndreas ReinerSpringer International Publishing