However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based ...
The 10-23 RNA cleaving DNAzyme has been shown to cleave any purine-pyrimidine (RY) junction under simulated physiological conditions. In this study, we systematically examine the DNAzymes relative activity against different RY combinations in order to determine the hierarchy of substrate core dinucleot...
A new and efficient DNA enzyme for the sequence-specific cleavage of RNA A new DNA enzyme, the "Bipartite DNAzyme", suitable for the sequence-specific cleavage of RNA, was obtained from a random DNA library by in vitro selection... AR Feldman,D Sen - 《Journal of Molecular Biology》 被...
DNAzymeRNA cleavageSingle Nucleotide Polymorphism (SNP)MALDI-TOF mass spectrometryAccurate and low-cost methods of genotyping of single nucleotide polymorphism (SNP) caused by single base-pair substitution are crucially important for prediction of hereditary diseases, design of individualized medicine, and ...
23 DNA enzyme”(DNAzymeAMBER 7 and 3DNA program packagesMg2+ cationsSummary This chapter contains sections titled: Introduction Results and Discussion Conclusion Acknowledgments Referencesdoi:10.1002/9781118092804.ch7Plashkevych, OleksandrBioorganic Chemistry Program, Department of Cell & Molecular Biology, ...
Efficiency of RNA cleavage; Sequences of DNAzyme and LNAzyme; Multiple-turnover of LNAzymes.VesterBirteLundbergLars BoSørensenMads D.BabuB. RavindraDouthwaiteStephenJournal of the American Chemical SocietyVESTER, B., LUNDBERG, L.B., SORENSEN, M.D., BABU, B.R., DOUTHWAITE, S., and ...
DNAzyme-based colorimetric sensing of lead (Pb(2+)) using unmodified gold nanoparticle probes. Novel functional oligonucleotides, especially DNAzymes with RNA-cleavage activity, have been intensively studied due to their potential applications in the... W Hui,B Li,L Jing,... - 《Nanotechnology》...
Site-specific cleavage of HCV genomic RNA and its cloned core and NS5B genes by DNAzyme. Journal of Gastroenterology & Hepatology. 2009 May; [Internet]. [cited 2010 July 29] 24 (5):872-878. Available from: http://web.ebscohost.com.ezproxy.drew.edu/ehost/detail?vid=1&hid=107&sid=...
1. The 10–23 DNAzyme, consisting of two binding arms that are complimentary to the target RNA sequence, is used as a probe to bind RNA substrate specifically, where the catalytic core of DNAzyme can cleave the scissile dinucleotide junction. The forward-cleaved RNA fragment, which hybridize ...
The 10–23 RNA cleaving DNAzyme has been shown to cleave any purine–pyrimidine (RY) junction under simulated physiological conditions. In this study, we systematically examine the DNAzymes relative activity against different RY combinations in order to determine the hierarchy of substrate core dinucle...