DNAzymes kineticsselectivity of RNA recognitionCleavage of biological mRNA by DNAzymes (Dz) has been proposed as a variation of oligonucleotide gene therapy (OGT). The design of Dz‐based OGT agents includes computational prediction of two RNA‐binding arms with low affinity (melting temperatures (...
RNA cleavageEngineering easy‐to 摗爄osensors with ultra﹍ow detection sensitivity remains a major challenge. Herein, we report a simple approach for creating such sensors through the use of an RNAヽleaving DNAzyme (RcD) and a strategy designed to significantly concentrate its cleavage product. ...
Cleavage reaction in anoxic environment and antioxidant ascorbate can be used to overcome the effect of oxidation. The findings provide insights for potential DNAzyme catalysis in early earth, and it further supports the similarity between Mg2+ and Fe2+ in enzyme catalysis....
However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based ...
Here, we report three catalytic form 8–17 DNAzyme crystal structures. 8–17 DNAzyme adopts a V-shape fold, and the Pb2+cofactor is bound at the pre-organized pocket. The structures with Pb2+and the modification at the cleavage site captured the pre-catalytic state of the RNA cleavage ...
e) Comparison of [1H,1H]-TOCSY fingerprint spectra of DNAc·RNA (cyan) and DNA5C·RNA2ʹF(black). Assignments for most affected nucleotides are indicated and show that in the absence of the catalytic loop, the respective peaks around the cleavage site shift into the region characteristic ...
DetectionofLarge Genomic RNA via DNAzyme-Mediated RNA Cleavage and Rolling Circle Amplification: SARS-CoV-2 as a Model Jimmy Gu, Amal Mathai, Connor Nurmi, Dawn White, Gurpreet Panesar, Prof. Dr. Deborah Yamamura, Prof. Dr. Cynthia Balion, Dr. Jonathan Gubbay, Prof. Dr. Karen Mo...
Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites Cairns MJ,King A,Sun LQ.Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites. Nucleic Acids Research...
Since Ce4+ is not an optimal metal for RNA cleavage (it is known to be better for DNA cleavage), the library actually used Ce3+ to evolve. This is quite an interesting DNAzyme, since it has a similar activity with all trivalent lanthanides (rate difference within four- fold from La3+...
M. Sequence-specific cleavage of RNA in the absence of divalent metal ions by a DNAzyme incorporating imidazolyl and amino functionalities. Nucleic Acids Res. 32, 1591–1601 (2004). Article CAS PubMed PubMed Central Google Scholar Wang, Y., Liu, E., Lam, C. H. & Perrin, D. M. ...