Cleavage of biological mRNA by DNAzymes (Dz) has been proposed as a variation of oligonucleotide gene therapy (OGT). The design of Dz‐based OGT agents includes computational prediction of two RNA‐binding arms with low affinity (melting temperatures (Tm) close to the reaction temperature of 37...
RNA cleavageEngineering easy‐to 摗爄osensors with ultra﹍ow detection sensitivity remains a major challenge. Herein, we report a simple approach for creating such sensors through the use of an RNAヽleaving DNAzyme (RcD) and a strategy designed to significantly concentrate its cleavage product. ...
Of all the technologies evaluated so far, DNAzyme-based heavy metal detectors and the MNAzyme-integrated PCR technologies have successfully made it to the market. The heavy metal detector, marketed by ANDalyze, Inc., provides water contamination testing solutions for Pb2+, Hg2+, Cu2+, Zn2+...
ensitive dual DNAzymes-based sensors designed by grafting self-blocked G-quadruplex DNAzymes to the substrates of metal ion-triggered DNA/RNA-cleaving DNAzyme In this strategy, the substrate strand of the DNA/RNA-cleaving DNAzyme was designed as an intramolecular stem–loop structure, and a G-...
The determined structure and identified metal-ion-binding sites of the precatalytic DNAzyme–RNA complex reveal that the basis of the DNA-mediated catalysis is an interplay among three factors: an unexpected, yet exciting molecular architecture; distinct conformational plasticity; and dynamic modulation ...
M. Sequence-specific cleavage of RNA in the absence of divalent metal ions by a DNAzyme incorporating imidazolyl and amino functionalities. Nucleic Acids Res. 32, 1591–1601 (2004). Article CAS PubMed PubMed Central Google Scholar Wang, Y., Liu, E., Lam, C. H. & Perrin, D. M. ...
F. A versatile endoribonuclease mimic made of DNA: Characteristics and applications of the 8-17 RNA-cleaving DNAzyme. ChemBioChem 2010, 11 (7), 866-879.Schlosser K, Li Y (2010) A Versatile Endoribonuclease Mimic Made of DNA: Characteristics and Applications of the 8–17 RNA-Cleaving DNAzyme...
Interestingly, Ce4+ is reported to be efficient for DNA cleavage instead of for RNA.52 The requirement of millimolar concentrations of lanthanides makes it analytically less interesting. We reason that the cleavage activity might be improved by adding a DNA scaffold (e.g., DNAzyme) to more ...
specificDNA/RNA-cleavingDNAzymeandaG-quadruplexDNAzyme.Inthisstrategy,thesubstrate strandoftheDNA/RNA-cleavingDNAzymewasdesignedasanintramolecularstem–loopstructure,and aG-richsequencewascagedinthedouble-strandedstemandcouldnotformcatalyticallyactive G-quadruplexDNAzyme.Themetalion-triggeredcleavageofthesubstrate...
C. DNA enzymes as potential therapeutics: towards clinical application of 10-23 DNAzymes. Expert Opin. Biol. Ther. 15, 689–711 (2015). Article CAS Google Scholar Krug, N. et al. Allergen-induced asthmatic responses modified by a GATA3-specific DNAzyme. N. Engl. J. Med. 372, 1987...