binding folded RNADNAzymes kineticsselectivity of RNA recognitionCleavage of biological mRNA by DNAzymes (Dz) has been proposed as a variation of oligonucleotide gene therapy (OGT). The design of Dz‐based OGT agents includes computational prediction of two RNA‐binding arms with low affinity (...
RNA cleavageEngineering easy‐to 摗爄osensors with ultra﹍ow detection sensitivity remains a major challenge. Herein, we report a simple approach for creating such sensors through the use of an RNAヽleaving DNAzyme (RcD) and a strategy designed to significantly concentrate its cleavage product. ...
So far, no RNA-cleaving DNAzymes specific for Cu2+ are known. In this work, a phosphorothioate (PS) RNA-containing library was used for in vitro ... PJJ Huang,J Liu - 《Analytical Chemistry》 被引量: 19发表: 2016年 A Complex RNA-Cleaving DNAzyme That Can Efficiently Cleave a Pyrimidi...
The determined structure and identified metal-ion-binding sites of the precatalytic DNAzyme–RNA complex reveal that the basis of the DNA-mediated catalysis is an interplay among three factors: an unexpected, yet exciting molecular architecture; distinct conformational plasticity; and dynamic modulation ...
F. A versatile endoribonuclease mimic made of DNA: Characteristics and applications of the 8-17 RNA-cleaving DNAzyme. ChemBioChem 2010, 11 (7), 866-879.Schlosser K, Li Y (2010) A Versatile Endoribonuclease Mimic Made of DNA: Characteristics and Applications of the 8–17 RNA-Cleaving DNAzyme...
2001. Suppression of plate- let-type 12-lipoxygenase activity in human erythroleukemia cells by an RNA-cleaving DNAzyme. Biochem Biophys Res Commun, 284, 1077-1082.Liu C,Cheng R,Sun LQ,et al.Suppression of platelet-type 12-lipoxygenase activity in human erythroleukemia cells by an RNA-...
Since Ce4+ is not an optimal metal for RNA cleavage (it is known to be better for DNA cleavage), the library actually used Ce3+ to evolve. This is quite an interesting DNAzyme, since it has a similar activity with all trivalent lanthanides (rate difference within four- fold from La3+...
specificDNA/RNA-cleavingDNAzymeandaG-quadruplexDNAzyme.Inthisstrategy,thesubstrate strandoftheDNA/RNA-cleavingDNAzymewasdesignedasanintramolecularstem–loopstructure,and aG-richsequencewascagedinthedouble-strandedstemandcouldnotformcatalyticallyactive G-quadruplexDNAzyme.Themetalion-triggeredcleavageofthesubstrate...
Targeted delivery of RNA-cleaving DNA enzyme (DNAzyme) to tumor tissue by transferrin-modified, cyclodextrin-based particles. Cancer Biol. Ther. 2004, 3, 641-650.Pun SH, Tack F, Bellocq NC, Cheng J, Grubbs BH, Jensen GS et al. Targeted delivery of RNA-cleaving DNA enzyme (DNAzyme) to...
Mutational analysis revealed a conservative character of the DNAzyme catalytic core that underpins the high structural requirements of the cleavage mechanism. A significant advantage of the described DNAzymes is that they are inactive at pH values close to physiological pH and under a wide range of ...