F. A DNA enzyme that cleaves RNA. Chem. Biol. 1, 223–229 (1994). Article CAS Google Scholar Uhlenbeck, O. C. A small catalytic oligoribonucleotide. Nature 328, 596–600 (1987). Article ADS CAS Google Scholar Brown, A. K., Li, J., Pavot, C. M. B. & Lu, Y. A lead-...
This reaction forms 2',5'-branched RNA and is analogous to the first step of in vivo RNA splicing, in which a ribozyme cleaves itself with formation of a branched intermediate. Unlike a natural ribozyme, the new DNA enzyme has no 2'-hydroxyl groups to aid in the catalytic mechanism. Our...
Furthermore the enzyme also hydrolyzes sphingomyelin to produce the respective lyso form. However, the enzyme shows hardly any activity on ceramides, indicating that it is completely different from the ceramidase (EC 3.5.1.23) reported previously. This enzyme, which is tentatively named sphingolipid...
et al. A new modified DNA enzyme that targets influenza virus A mRNA inhibits viral infection in cultured cells. FEBS Lett. 560, 69–74 (2004). Article CAS PubMed Google Scholar Vester, B. et al. LNAzymes: incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage....
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. ...
It also cleaves RNA weakly at UC, CU, AC, and AU sites in single-stranded or weakly paired regions. Finally, we found that APE1 can reduce the ability of the Dicer enzyme to process premiRNAs in vitro. Overall, this study has revealed some previously unknown biochemical properties of APE...
An enzyme that cleaves protein at positions within the chain. Formally, the enzymes are peptidyl-peptide hydrolases, often referred to as proteinases or proteolytic enzymes. endopeptidase 24.11 See NEP #1. endophilin Family of proteins with a BAR domain involved in regulating clathrin-mediated endocytos...
An enzyme that cleaves 1,3-dicarbonyl compounds by incorporating molecular oxygen into the substrate in amounts equimolar to the amount of substrate cleave... G Straganz,L Brecker,A Glieder 被引量: 0发表: 2004年 MicroRNA-135b has a neuroprotective role via targeting of beta-site APP-cleavin...
DNA:DNA:RNA triplexes that are formed through Hoogsteen base-pairing of the RNA in the major groove of the DNA duplex have been observed in vitro, but the extent to which these interactions occur in cells and how they impact cellular functions remains elusive. Using a combination of bioinforma...
When their recognition site is unmodified (i.e., unmethylated), the enzyme cleaves (“restricts”) the sequence. When only one strand of the recognition sequence is methylated (i.e., hemimethylated), the enzyme methylates ...